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PCR Mutation Detection Protocols
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Main description:

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method.

Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.


Feature:

Fully updates the popular previous edition
Offers cutting-edge techniques, such as those for microarrays, with the underlying basis of the method for successful implementation
Features key tips from the expert contributors drawing upon their extensive experience


Back cover:

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method.

Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.


Contents:

1. Conformation Sensitive Capillary Electrophoresis (CSCE)
Emma Jane Ashton

2. Conformation Sensitive Gel Electrophoresis
Marian Hill

3. Denaturing HPLC (dHPLC) for Mutation Screening
Mike Mitchell and Jacky Cutler

4. In situ Detection of Human Papillomavirus DNA after PCR-Amplification
Gerard J. Nuovo

5. LATE-PCR and Allied Technologies: Real-Time Detection Strategies for Rapid, Reliable Diagnosis from Single Cells
Kenneth E. Pierce and Lawrence J. Wangh

6. Long PCR Amplification of Human Genomic DNA
Stephen Keeney

7. Human Papilloma Virus Strain Detection Utilizing Custom-Designed Oligonucleotide Microarrays
Duncan Ayers, Mark Platt, Farzad Javad, and Philip J.R. Day

8. Multiplex Ligation-Dependent Probe Amplification (MLPA®) for the Detection of Copy Number Variation in Genomic Sequences
Petra G.C. Eijk - van Os and Jan P. Schouten

9. Screening for Genomic Rearrangements by Multiplex PCR/Liquid Chromatography (MP/LC)
Houdayer Claude, Dehainault Catherine, Gauthier-Villars Marion, and Stoppa-Lyonnet Dominique

10. Mutation Surveyor: Software for DNA Sequence Analysis
Jayne A.L. Minton, Sarah E. Flanagan, and Sian Ellard

11. Non-Invasive Prenatal Diagnosis (NIPD)
Cathy Meaney and Gail Norbury

12. Automated DNA Sequencing
Yvonne Wallis and Natalie Morrell

13. Phylogenetic Microarrays for Cultivation-Independent Identification and Metabolic Characterization of Microorganisms in Complex Samples
Alexander Loy, Michael Pester, and Doris Steger

14. Prenatal Detection of Chromosome Aneuploidy by Quantitative-Fluorescence PCR
Kathy Mann, Erwin Petek, and Barbara Pertl

15. Use of Robotics in High-Throughput DNA Sequencing
Stephen Keeney

16. Detection of Factor V Leiden and Prothrombin c.20210G>A Allele by Roche Diagnostics LightCycler®
Peter C. Cooper

17. RT-PCR for the Detection of Translocations in Bone and Soft Tissue Tumors in Formalin-Fixed Paraffin-Embedded Tissues
Ann Williams and D. Chas Mangham

18. Detection of Minimal Residual Disease in Leukaemia by RT-PCR
Joanne Mason and Mike Griffiths

19. Mutation Detection by Southern Blotting
Gillian Mellars and Keith Gomez


PRODUCT DETAILS

ISBN-13: 9781607619468
Publisher: Springer (Humana Press)
Publication date: October, 2010
Pages: 275
Weight: 1660g
Availability: POD
Subcategories: General Issues, Genetics
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