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Main description:
Due to their rare combination of high chemical stability, exceptional optical and electrical properties, high surface-to-volume ratio, and high aspect ratio, carbon nanotubes (CNTs) have made an enormous impact on materials science, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: Methods and Protocols provides reliable, consistent protocols on the application of CNTs in molecular biology-related fields. These are of vital importance, as the commercially available CNTs differ in purity, agglomeration state, as well as length and diameter distribution, all of which have a profound influence on the dispersability and surface properties of the tubes. The volume contains detailed sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, provided by expert researchers from various fields. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Carbon Nanotubes: Methods and Protocols serves to contribute to the achievement of common standards and helps researchers to avoid discrepancies in future biology-related CNT studies.
Contents:
Part I: Functionalization
1. Non-Covalent Attachment of Proteins to Single-Walled Carbon Nanotubes
Luis F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan, David W. Schmidtke, Daniel E. Resasco, and Roger G. Harrison
2. Covalent Conjugation of Multi-Walled Carbon Nanotubes with Proteins
Changqing Yi, Suijian Qi, Dawei Zhang, and Mengsu Yang
3. Covalently Linked Deoxyribonucleic Acid with Multi-Walled Carbon Nanotubes: Synthesis and Characterization
Weiwei Chen, Changqing Yi, Tzang Chi-Hung, Shuit-Tong Lee, and Mengsu Yang
4. Temperature and pH-Responsive 'Smart' Carbon Nanotube Dispersions
Dan Wang and Liwei Chen
Part II: Toxicity
5. Effects of Carbon Nanotubes on the Proliferation and Differentiation of Primary Osteoblasts
Dawei Zhang, Changqing Yi, Suijian Qi, Xinsheng Yao, and Mengsu Yang
6. Carbon Nanotube Uptake and Toxicity in the Brain
Leying Zhang, Darya Alizadeh, and Behnam Badie
7. In vitro and in vivo Biocompatibility Testing of Functionalized Carbon Nanotubes
Gianni Ciofani, Vittoria Raffa, Orazio Vittorio, Alfred Cuschieri, Tommaso Pizzorusso, Mario Costa, and Giuseppe Bardi
8. Real-time Monitoring of Cellular Responses to Carbon Nanotubes
Qingxin Mu, Shumei Zhai, and Bing Yan
9. Reducing Nanotube Cytotoxicity Using a Nano-Combinatorial Library Approach
Qiu Zhang, Hongyu Zhou, and Bing Yan
10. DNA Damage by Carbon Nanotubes Using the Single Cell Gel Electrophoresis Technique
Olga Zeni and Maria Rosaria Scarfi
Part III: Trafficking
11. Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry
Khuloud T. Al-Jamal and Kostas Kostarelos
12. Cell Trafficking of Carbon Nanotubes Based on FluorescenceDetection
Monica H. Lamm and Pu Chun Ke
13. Carbon Nanotubes as Intracellular Carriers for Multidrug Resistant Cells Studied by Capillary Electrophoresis-Laser Induced Fluorescence (CE-LIF)
Ruibin Li, Hanfa Zou, Hua Xiao, and Renan Wu
Part IV: Scaffolds
14. Carbon Nanotube-Based Neurochips
Moshe David-Pur, Mark Shein, and Yael Hanein
15. Effect of Carbon Nanotubes on HepG2 Adhesion and Spreading
Suijian Qi, Changqing Yi, Dawei Zhang, and Mengsu Yang
Part V: Biosensors
16. Enzymatic Detection Based on Carbon Nanotubes
Martin Pumera
17. Carbon Nanotube Biosensors Based on Electrochemical Detection
Martin Pumera
18. Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors
Cristina C. Cid, Jordi Riu, Alicia Maroto, and F. Xavier Rius
19. Detection of Biomarkers with Carbon Nanotube-Based Immunosensors
Samuel Sanchez, Esteve Fabregas, and Martin Pumera
20. Carbon Nanotube Biosensors with Aptamers as Molecular Recognition Elements
Hye-Mi So, Dong-Won Park, Hyunju Chang, and Jeong-O Lee
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: March, 2010
Pages: 250
Weight: 768g
Availability: Available
Subcategories: Biochemistry
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