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MORE ABOUT THIS BOOK
Main description:
This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes. The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches. Thechoiceofchapterswasmadetakingintoaccounttwopoints:First,manyofthe techniquesthathavebeen usedfor sometimehave beenupgraded duringthe pastfew years given the greater availability of a variety of products (i.e. cytokines, chemokines, monoclonal antibodies), of refined technical devices (i.e. novel cell culture and cell analysis equipments), and the development of novel instrumentation (i.e. multipa- metric flow cytometers, confocal microscopes). Therefore, in several chapters ‘‘old techniques’’, which remain fundamental to T cell immunology, are described in their ‘‘modern’’ versions. Secondly, the technical advancement has generated the possibility to establish novel assays to investigate T cell physiology. This is reflected in the chapters which describe the protocols that allow use of these modern approaches. The preparation of this book has required participation of several scientists, all leading experts in their respective fields. Without their enthusiastic participation, this work would not have been possible. Therefore, I thank all authors for their contri- tionsandacceptallcriticismformissingparts,orinformationordetails,forwhichonly I am responsible. I hope these protocols will be useful for young investigators who approach for the first time the complex field of immunology and for those more experienced scientists who look for concise and efficacious descriptions of novel methods.
Feature:
Provides an easily accessible reference volume for cell-based phenotypic screening, written by leading researchers in the field
Covers a wide range of cell-based assay formats and systems
Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and approaches to data analysis
Comprehensive guide to developing, running, and analyzing cell-based assays
Back cover:
With a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls.
Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.
Contents:
Chapter 1: Analysis of Frequency and Phenotype of Antigen-Specific T Cells
Angus Stock and Vincenzo Cerundolo
Chapter 2: B Cell Helper Assays
Sergio Abrignani, Elena Tonti, Giulia Casorati, and Paolo Dellabona
Chapter 3: Trans-Kingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing
Thu A. Nguyen and Johannes H. Fruehauf
Chapter 4: Flow Cytometry and Cell Activation
Sonia Gavasso
Chapter 5: Investigating T Cells by Polychromatic Flow Cytometry
Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza
Chapter 6: Generation of Human T Cell Clones
Sabrina Mariotti and Roberto Nisini
Chapter 7: Limiting Dilution Analysis of Antigen-Specific T cells
Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan
Chapter 8: T Cell Epitope-Mapping by Cytokine Gene Expression Assay
Maurizio Provengano and Giulio C. Spagnoli
Chapter 9: Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications
Wilco de Jager, Berent Prakken, and Ger T. Rijkers
Chapter 10: Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE
Mahima Swamy and Wolfgang W.A. Schamel
Chapter 11: Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation
Paul Zajac
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: December, 2008
Pages: 166
Weight: 657g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry, Immunology
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CUSTOMER REVIEWS
From the reviews of the second edition:
"A great collection of protocols, some totally new and some upgraded … to study the biology of this fascinating cell is wellcome by the scientific community. … Thanks to the generation of … PFC, is now at the hands of those scientists that want to advance our understanding of T cells biology. … the book will be of enourmous help both to beginners and to experienced scientists is well underlining the esprit of the book, as I showed with the three examples illustrated." (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)