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MORE ABOUT THIS BOOK
Main description:
This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.
Contents:
1. Genome Editing by Targeted Chromosomal Mutagenesis
Dana Carroll
2. piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells
Stephen J. Pettitt, E-Pien Tan, and Kosuke Yusa
3. Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy
Jonathan M. Geisinger and Michele P. Calos
4. Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA
Faisal Reza and Peter M. Glazer
5. Genome Engineering Using Adeno-Associated Virus (AAV)
Rob Howes and Christine Schofield
6. Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization
Ryo Takeuchi, Michael Choi, and Barry L. Stoddard
7. Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform
Tomas Cermak, Colby G. Starker, and Daniel F. Voytas
8. Ligation-Independent Cloning (LIC) Assembly of TALENs
Jonathan L. Schmid-Burgk, Tobias Schmidt, and Veit Hornung
9. Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications
Sandrine Boissel and Andrew M. Scharenberg
10. Genome Engineering Using CRISPR-Cas9 System
Le Cong and Feng Zhang
11. Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases
Shondra M. Pruett-Miller and Gregory D. Davis
12. Endogenous Gene Tagging with Fluorescent Proteins
John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, and Dmitry Malkov
13. Silencing Long Non-Coding RNAs with Genome-Editing Tools
Tony Gutschner
14. Gene Editing Using ssODNs with Engineered Endonucleases
Fuqiang Chen, Shondra M. Pruett-Miller, and Gregory D. Davis
15. Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases
Kunitoshi Chiba and Dirk Hockemeyer
16. Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells
Matthew Porteus
17. Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models
Victoria M. Bedell and Stephen C. Ekker
18. Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection
Takehito Kaneko and Tomoji Mashimo
19. Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains
Takehito Kaneko
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2014
Pages: 335
Weight: 899g
Availability: Available
Subcategories: Genetics
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