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Main description:
A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contents:
Isothermal Titration Calorimetry
Edwin A. Lewis and Kenneth P. Murphy
Direct Optical Detection of Protein-Ligand Interactions
Frank Gesellchen, Bastian Zimmermann, and Friedrich W. Herberg
Label-Free Detection of Protein-Ligand Interactions by the Quartz Crystal Microbalance
Andreas Janshoff and Claudia Steinem
Measurement of Solvent Accessibility at Protein-Protein Interfaces
Jeffrey G. Mandell, Abel Baerga-Ortiz, Arnold M. Falick, and Elizabeth A. Komives
Hydrophobic Interaction Chromatography: Harnessing Multivalent Protein-Surface Interactions for Purification Procedures
Herbert P. Jennissen
Sedimentation Velocity Method in the Analytical Ultracentrifuge for the Study of Protein-Protein Interactions
Claus Urbanke, Gregor Witte, and Ute Curth
Protein-Ligand Interaction Probed by Time-Resolved Crystallography
Marius Schmidt, Hyotcherl Ihee, Reinhard Pahl, and Vukica Srajer
X-Ray Crystallography of Protein-Ligand Interactions
Ilme Schlichting
Combined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in Metalloproteins
Richard W. Strange and S. Samar Hasnain
NMR Studies of Protein-Ligand Interactions
Till Maurer
Probing Heme Protein-Ligand Interactions by UV/Visible Absorption Spectroscopy
Karin Nienhaus and G. Ulrich Nienhaus
Ultrafast Time-Resolved IR Studies of Protein-Ligand Interactions
Manho Lim and Philip A. Anfinrud
Monitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference Spectroscopy
Carsten Koetting and Klaus Gerwert
Proteins in Motion: Resonance Raman Spectroscopy as a Probe of Functional Intermediates
Uri Samuni and Joel M. Friedman
Fluorescence Polarization/Anisotropy Approaches to Study Protein-Ligand Interactions: Effects of Errors and Uncertainties
David M. Jameson and GaborMocz
Ligand Binding With Stopped-Flow Rapid Mixing
Mark S. Hargrove
Circular Dichroism Spectroscopy for the Study of Protein-Ligand Interactions
Alison Rodger, Rachel Marrington, David Roper, and Stuart Windsor
High-Throughput Screening of Interactions Between G Protein-Coupled Receptors and Ligands Using Confocal Optics Microscopy
Lenka Zemanova, Andreas Schenk, Martin J. Valler, G. Ulrich Nienhaus, and Ralf Heilker
Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics
H. Peter Lu
Application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding
Theodore L. Hazlett, Qiaoqiao Ruan, and Sergey Y. Tetin
Atomic Force Microscopy Measurements of Protein-Ligand Interactions on Living Cells
Robert H. Eibl and Vincent T. Moy
Computer Simulation of Protein-Ligand Interactions: Challenges and Applications
Sergio A. Hassan, Luis Gracia, Geetha Vasudevan, and Peter J. Steinbach
Force Probe Molecular Dynamics Simulations
Helmut Grubmuller
Study of Ligand-Protein Interactions by Means of Density Functional Theory and First-Principles Molecular Dynamics
Carme Rovira
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 584
Weight: 848g
Availability: Available
Subcategories: Biochemistry
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