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Main description:
Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies.
Contents:
Immunization Strategies for Antibody Production
Robert Burns
Synthetic Peptides as Antigens for Antibody Production
David C. Hancock and Nicola J. O'Reilly
Production of Polyclonal Antibodies in Rabbits
David C. Hancock and Nicola J. O'Reilly
Making Hybridomas
Robert Burns
Growing Hybridomas
Gary Entrican and Gareth Young
Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments
Michael Hust and Stefan Dubel
Purification of Antibodies and Preparation of Antibody Fragments
Graham D. Kemp
Conjugation of Antibodies to Alkaline Phosphatase
G. Brian Wisdom
Conjugation of Antibodies to Horseradish Peroxidase
G. Brian Wisdom
Conjugation of Antibodies to Fluorescein or Rhodamine
G. Brian Wisdom
Conjugation of Antibodies to Biotin
James D. Hirsch and Rosaria P. Haugland
Preparation of Colloidal Gold Probes
David Hughes
Immunogold Probes in Light Microscopy
David Hughes
Immunoelectron Microscope Techniques in Plant Virus Diagnosis
Daphne M. Wright
Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay
William Jordan
Competitive Enzyme-Linked Immunosorbent Assay
William Jordan
Immunoblotting Techniques
Barbara Magi and Sabrina Liberatori
Epitope Mapping
Glenn E. Morris
Generation and Testing of Immunoaffinity Columns
Weilin L. Shelver
Immunocapture-Polymerase Chain Reaction
Vincent Mulholland
Magnetic Cell Sorting
Maciej Zborowski and Jeffrey J. Chalmers
Cell Enrichment and Immunochemical Staining
Oscar R. Lara and Jeffrey J. Chalmers
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 328
Weight: 484g
Availability: Available
Subcategories: Biochemistry
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