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Main description:
Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.
Contents:
Part I: Genetic, Structural Biology, Transcriptional and Post-Transcriptional Analysis of Opioid Receptors
1. Overview of Genetic Analysis of Human Opioid Receptors
Santi M. Spampinato
2. Computational Structural Biology of Opioid Receptors
Davide Provasi
3. Analysis of Epigenetic Mechanisms Regulating Opioid Receptor Gene Transcription
Cheol Kyu Hwang, Yadav Wagley, Ping-Yee Law, Li-Na Wei, and Horace H. Loh
4. Renilla Luciferase Reporter Assay to Study 3'UTR-Driven Post-Transcriptional Regulations of OPRM1
Gabriele Vincelli and Andrea Bedini
5. High-Throughput Gene Expression Profiling of Opioid-Induced Alterations in Discrete Brain Areas
Michal Korostynski, Marcin Piechota, Slawomir Golda, and Ryszard Przewlocki
Part II: Cellular Detection and Analysis of Opioid Receptors
6. Real-Time Imaging of Mu Opioid Receptors by Total Internal Reflection Fluorescence Microscopy
Cristina Roman-Vendrell and Guillermo Ariel Yudowski
7. In Vivo Techniques to Investigate the Internalization Profile of Opioid Receptors
Amynah A. Pradhan, Vivianne L. Tawfik, Alycia F. Laboy, and Gregory Scherrer
8. Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)
Monica Baiul
a
9. Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-Visual Arrestins
Andrea Bedini
10. Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR)
Chongguang Chen, Peng Huang, and Lee-Yuan Liu-Chen
11. Detection of Mu Opioid Receptor (MOPR) and Its Glycosylation in Rat and Mouse Brains by Western Blot with Anti-C, an Affinity-Purified Polyclonal Anti-MOPR Antibody
Peng Huang, Chongguang Chen, and Lee-Yuan Liu-Chen
12. Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues
Yvonne Schmidt and Halina Machelska
Part III: Analysis of Signaling Events Modulated by Opioid Receptors
13. [35S]GTPgS-Autoradiography for Studies of Opioid Receptor Functionality
Alfhild Groenbladh and Mathias Hallberg
14. Fluorescence-Based, High-Throughput Assays for -Opioid Receptor Activation Using Membrane Potential-Sensitive Dye
Alisa Knapman and Mark Connor
15. Analysis of Potassium and Calcium Imaging to Assay the Function of Opioid Receptors
Viola Spahn, Dinah Nockemann, and Halina Machelska
16. Electrophysiological Patch Clamp Assay to Monitor the Action of Opioid Receptors
Viola Spahn, Dinah Nockemann, a
nd Halina Machelska
Part IV: Model Systems to Studying Opioid Receptor-Mediated Functions
17. Skin-Nerve Preparation to Assay the Function of Opioid Receptors in Peripheral Endings of Sensory Neurons
Rabih Moshourab, Yvonne Schmidt, and Halina Machelska
18. Mechanical Nociception Measurement in Mice and Rats with Automated Von Frey Equipment
Gabriele Campana and Roberto Rimondini
19. Detecting Zinc Release Induced by Mu-Opioid Receptor Agonists in Brain Slices
Maria Rodriguez-Munoz, Pilar Sanchez-Blazquez, Concha Bailon, and Javier Garzon
20. Opioid Receptors: Methods for Detection and Their Modes of Actions in the Eye
Shahid Husain
21. Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment
Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai, and Paola Sacerdote
22. Measurement of Macrophage Toll Like Receptor 4 Expression After Morphine Treatment
Mara Castelli, Alberto Panerai, Paola Sacerdote, and Silvia Franchi
23. The Role of Opioid Receptors in Migration and Wound Recovery In Vitro in Cultured Human Keratinocytes and Fibroblasts
Mei Bigliardi-Qi and Paul L. Bigliardi
Part V: Behavioral Effects Mediated by Opioid Receptors
24. Role of Opioid Receptors in the Reinstatemen
t of Opioid-Seeking Behavior: An Overview
Liana Fattore, Paola Fadda, Mary Silvia Antinori, and Walter Fratta
25. Analysis of Opioid-Seeking Reinstatement in the Rat
Liana Fattore, Paola Fadda, Mary Tresa Zanda, and Walter Fratta
26. Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors
Roberto Rimondini and Gabriele Campana
27. Evaluation of Social and Non-Social Behaviors Mediated by Opioids in Mouse Pups
Francesca R. D'Amato
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2014
Pages: 310
Weight: 8678g
Availability: Available
Subcategories: Neuroscience, Pharmacology
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