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MORE ABOUT THIS BOOK
Main description:
Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.
Contents:
1. In Vitro Invasion Assay Using MatrigelTM - a Reconstituted Basement Membrane Preparation
Debbie M S Hall and Susan A Brooks
2. Single Cell and Spheroid Collagen Type I Invasion Assay
Olivier De Wever , An Hendrix, Astrid De Boeck, Frank Eertmans, Wendy Westbroek, Geert Braems, Marc Bracke
3. Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells
Deepashree Bapu, Munira Khadim and Susan A. Brooks
4. Small Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin under Physiological Flow Conditions
Ulrich Richter and Udo Schumacher
5. Adhesion of Tumour Cells to Matrices and Endothelium
Clara M Yates, Helen M McGettrick, Gerard B Nash, and G. Ed Rainger
6. Cell Aggregation Assays
Delphine Debruyne, Tom Boterberg, and Marc E. Bracke
7. Chick Heart Invasion Assay
Marc E. Bracke, Virinder S. Parmar, Anthony L. DePass, Christian V. Stevens, Barbara W. Vanhoecke, and Marc M. Mareel
8. Computer Simulation of the Metastatic ProgressionGero Wedemann, Anja Bethge, Volker Haustein, and Udo Schumacher
9. Theoretical Considerations In Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice
Sue A. Watson and Rajendra Kumari
10. Syngeneic Murine Metastasis Models: B16 MelanomaRaffaella Giavazzi and Alessandra Decio
11. Imageable Clinically-relevant Mouse Models of Metastasis
Robert M. Hoffman
12. Imaging Metastatic Cell Trafficking at the Cellular Level in vivo with Fluorescent Proteins
Robert M. Hoffman
13. Ultrasound Techniques for the Detection of Tumours and Metastases in Small Animals
Michael Didie and Wolfram-Hubertus Zimmermann
14. The PFP/RAG2 Double Knock Out Mouse in Metastasis Research: Small Cell Lung Cancer and Prostate Cancer
Imke Muller and Sebastian Ullrich
15. Ultrasound Guided Intracardial Injection and In-vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Haematogenous Metastases
Johannes Salamon and Kersten Peldschus
16. Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer
Kersten Peldschus and Harald Ittrich
17. Spontaneous and Experimental Metastasis Models: Nude Mice
Janet E Price
18. Identifying the Origin and Phenotype of Cells in Tumour Xenografts
Rosemary Jeffery, Pooja Seedhar, and Richard Poulsom
PRODUCT DETAILS
Publisher: Springer (Springer-Verlag New York Inc.)
Publication date: October, 2013
Pages: 250
Weight: 696g
Availability: Available
Subcategories: General Issues, Oncology
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