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Main description:
In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology (TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.
Contents:
T-Cell Trafficking: Methods and Protocols
Edited by Federica M. Marelli-Berg and Sussan Nourshargh
Table of Contents
Preface
Contributors
Introductory review:
1. How T cells find their way around
Alf Hamann
Migration of T cells in vitro
2. Live imaging of leukocyte-endothelium interactions
Olga Barreiro, Francisco Sanchez-Madrid, and Maria Yanez-Mo
3. Leukocyte adhesion under haemodynamic flow conditions
Charlotte Lawson, Marlene Rose and Sabine Wolf
4. Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells
Helen M. McGettrick PhD, Chris D. Buckley PhD, G. Ed Rainger PhD
and Gerard B. Nash PhD
5. Discriminating between the paracellular and transcellular routes of diapedesis
Eva Cernuda-Morollon, Severine Gharbi, Jaime Millan
6. Monitoring RhoGTPase activity in lymphocytes
Marouan Zarrouk, David Killock, Aleksandar Ivetic
7. Visualisation of Signalling in Immune Cells
Leo M. Carlin, Konstantina Makrogianneli, Melanie Keppler, Gilbert O. Fruhwirth and Tony Ng
8. Methods for quantisation of lymphocyte chemotaxis and fugetaxis
Fabrizio Vianello, Elda Righi and Mark C. Poznansky
9. Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes
Anna Korniejewska, Malcolm Watson, Stephen Ward
10. Transfection of indoleamine 2,3 dioxygenase in primary endothelial cells
Petros XE Mouratidis, Andrew J T George
Migration of T cells in vivo
11. Visualisation of lymphoid organ development
Henrique Veiga-Fernandes, Katie Foster, Amisha Patel, Mark Coles, and Dimitris Kioussis
12. Single Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy
Thorsten R. Mempel
13. Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy
Owain R. Millington, James M. Brewer, Paul Garside, and Pasquale Maffia
14. Applying an adaptive watershed to the tissue cell quantification during T-cell migration and embryonic development
D. Zhu, S. Jarmin, A. Ribeiro, F. Prin, S.Q. Xie, K. Sullivan, J. Briscoe, A.P. Gould, F.M. Marelli-Berg & Y. Gu
Monitoring T cell migration in human diseases
15. Identifying homing interactions in T cell traffic in human disease
Patricia F. Lalor, Stuart M. Curbishley and David H. Adams
16. Tracking antigen experienced effector T cells in vitro and in vivo
Claire L. Gorman, Claudia Monaco, Enrico Ammiratti, Anna-Chiara Vermi, Federica Marelli-Berg and Andrew P. Cope
17. Pre-clinical testing of strategies for therapeutic targeting of human T cell trafficking in vivo
Caroline Coisne and Britta Engelhardt
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2010
Pages: 302
Weight: 1660g
Availability: Available
Subcategories: General Issues, Immunology
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