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MORE ABOUT THIS BOOK
Main description:
Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.
Contents:
1. Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells
Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko
2. Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo
Sidney B. Cambridge
3. Optochemical Activation of Kinase Function in Live Cells
Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters
4. Photoswitching of Cell Surface Receptors Using Tethered Ligands
Andreas Reiner and Ehud Y. Isacoff
5. Photocontrol of AMPA Receptors with a Photochromic Ligand
Martin Sumser and Philipp Stawski
6. Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy
Nina Wittenmayer
7. Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins
Lauren R. Polstein and Charles A. Gersbach
8. Manipulation of Plasma Membrane Phosphoinositides using Photoinduced Protein-protein Interactions
Olof Idevall-Hagren and Pietro DeCamilli
9. A Roadmap to Applying Optogenetics in Neuroscience
Consuelo Fois, Pierre-Hugues Prouvot, and Albrecht Stroh
10. Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors
Marion Mutter, Natalia Swietek & Thomas A. Munch
11. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity
Wagner Steuer Costa, Jana Liewald, Alexander Gottschalk
12. Structural Basis of Photoswitching in Fluorescent Proteins
Chenxi Duan, Virgile Adam, Martin Byrdin and Dominique Bourgeois
13. Using Photoactivatable GFP to Track Axonal Transport Kinetics
Archan Ganguly and Subhojit Roy
14. In Vivo Cell Tracking Using PhOTO Zebrafish
William P. Dempsey, Hanyu Qin, Periklis Pantazis
15. In vivo Optogenetics for Light-induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein
Cathleen Teh and Vladimir Korzh
16. Photoactivatable Fluorescent Proteins for Super-Resolution Microscopy
Yuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus
17. pcSOFI as a Smart Label-based Super resolution Microscopy Technique
Benjamien Moeyaert and Peter Dedecker
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2014
Pages: 245
Weight: 7609g
Availability: Available
Subcategories: Neuroscience
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