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Main description:
Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson's disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.
Contents:
Part I: Biochemical, Proteomic, and Computational Tools
1. Detection of Cell Surface Dopamine Receptors
Jiping Xiao and Clare Bergson
2. Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells
Peiying Yu, Van Anthony Villar, and Pedro A. Jose
3. Methods of Dopamine Research in Retina Cells
Ana Lucia Marques Ventura, Fernando Garcia de Mello, and Ricardo Augusto de Melo Reis
4. Capture of D2 Dopamine Receptor Signaling Complexes in Striatal Cells for Mass Spectrometry Proteomic Analysis
Nadine Kabbani and Jacob C. Nordman
5. Modeling Spatial Aspects of Intracellular Dopamine Signaling
Kim Blackwell, Lane J. Wallace, BoHung Kim, Rodrigo Oliveira, and Wonryull Koh
Part II: Cellular Imaging
6. A Biophysical Approach for the Study of Dopamine Receptor Oligomerization
Sylwia Lukasiewicz, Agata Faron-Gorecka, and Marta Dziedzicka-Wasylewska
7. Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET (SRET) Technology
Gemma Navarro, Peter J. McCormick, Josefa Mallol, Carme Lluis, Rafael Franco, Antoni Cortes, Vicent Casado, Enric I. Canela, and Sergi Ferre
8. BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling
Stefano Espinoza, Bernard Masri, Ali Salahpour, and Raul R. Gainetdinov
9. Dopaminergic Regulation of Dendritic Calcium: Fast Multi-Site Calcium Imaging
Wen-Liang Zhou, Katerina D. Ikonomu, Shaina M. Short, and Srdjan D. Antic
Part III: Genetic Manipulation in Cells and Organisms
10. Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosolic End of Transmembrane Region 6
Bianca Plouffe and Mario Tiberi
11. A Molecular Genetic Approach to Uncovering the Differential Functions of Dopamine D2 Receptor Isoforms
Yanyan Wang, Toshikuni Sasaoka, and Mai T. Dang
12. Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish
Wendy Boehmler, Jessica Petko, Victor A. Canfield, and Robert Levenson
13. Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila
Eswar Prasad R. Iyer, Srividya Chandramouli Iyer, and Daniel N. Cox
Part IV: Electrochemical, Physiological, and Behavioral Analysis
14. Regulation of Dopamine Transporter Expression by Neuronal Activity
Shalini Padmanabhan, Thach Pham, and Balakrishna M. Prasad
15. Monitoring Somatodendritic and Axonal Dopamine Release Using Fast-Scan Cyclic Voltammetry in Brain Slices
Jyoti C. Patel and Margaret E. Rice
16. Real-Time Chemical Measurements of Dopamine Release in the Brain
James G. Roberts, Leyda Z. Lugo-Morales, Philip L. Loziuk, and Leslie A. Sombers
17. The MPTP/Probenecid Model of Progressive Parkinson's Disease
Anna R. Carta, Ezio Carboni, and Saturnino Spiga
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: January, 2013
Pages: 342
Weight: 7538g
Availability: Available
Subcategories: Neurology, Neuroscience
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