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MORE ABOUT THIS BOOK
Main description:
This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.
Feature:
Includes cutting-edge methods and protocols for the study of Staphylococci genetic manipulation
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.Contents:
1. Understanding Staphylococcal Nomenclature
Christina N. Krute and Jeffrey L. Bose
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2. Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus
Marat R. SadykovÂ
3. Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci
Ralph Bertram
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4. De Novo Assembly of Plasmids Using Yeast Recombinational Cloning
Ameya Mashruwala and Jeffrey M. Boyd
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5. Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products
Justin A. Thornton
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6. Method for Preparation and Electroporation of S. aureus and S. epidermidis
Melinda R. Grosser and Anthony R. Richardson
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7. Rapid Isolation of DNA from Staphylococcus
Kelsey L. Krausz and Jeffrey L. Bose
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8. Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method
Kelsey L. Krausz and Jeffrey L. Bose
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9. Bacteriophage Transduction in Staphylococcus aureus
Michael E. Olson
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10. Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus
Katherine L. Maliszewski
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11. Conjugative Transfer in Staphylococcus aureus
Cortney R. Halsey and Paul D. Fey
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12. Allelic Exchange
McKenzie K. Lehman, Jeffrey L. Bose, and Kenneth W. Bayles
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13. Creation of Staphylococcal Mutant Libraries Using Transposon Tn917
Kelly C. Rice
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14. Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using Bursa aurealis
Vijaya Kumar Yajjala, Todd J. Widhelm, Jennifer L. Endres, Paul D. Fey, and Kenneth W. Bayles
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15. Chemical and UV Mutagenesis
Jeffrey L. Bose
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16. Pulsed Field Gel Electrophoresis
Batu K. Sharma-Kuinkel, Thomas H. Rude, and Vance G. Fowler, Jr
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17. RNA-Sequencing of Staphylococcus aureus Messenger RNA
Ronan K. Carroll, Andy Weiss, and Lindsey N. Shaw
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18. Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression
April M. Lewis and Kelly C. Rice
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19. Electrophoretic Mobility Shift Assays
Sarah E. Rowe and James P. O’Gara
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20. Rapid Amplification of cDNA Ends for RNA transcript Sequencing in Staphylococcus
Eric Miller
PRODUCT DETAILS
Publisher: Springer (Springer New York)
Publication date: October, 2015
Pages: 180
Weight: 565g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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