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Main description:
This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and mitochondrial DNA copy number, the latest technologies for modifying the mitochondrial genome and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.
Contents:
Part I: Detection of Mitochondrial DNA Mutations and Mitochondrial DNA Damage
1. Comprehensive Mitochondrial Genome Analysis By Massively Parallel Sequencing
Meagan E. Palculict, Victor Wei Zhang, Lee-Jun Wong, and Jing Wang
2. Analysis Of Translesion DNA Synthesis By The Mitochondrial DNA Polymerase g
William C. Copeland, Rajesh Kasiviswanathan, and Matthew J. Longley
3. Quantification Of DNA Damage By Real-Time Qpcr
Wei Wang, Katja Scheffler, Ying Esbensen, And Lars Eide
4. Quantitation Of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule
PCR
Yevgenya Kraytsberg, Xinhong Guo, Saisai Tao, Alexandra Kuznetsov, Catherine Mclean, Daniel Ehrlich, Evan Feldman, Igor Dombrovsky, Deye Yang, Gregory J. Cloutier, Carmen Castaneda-Sceppa, And Konstantin Khrapko
Part II: Visualization of Mitochondrial DNA and Mitochondrial DNA Nucleoids
5. A Single-Cell Resolution Imaging Protocol Of Mitochondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity
Laurent Chatre, Benjamin Montagne and Miria Ricchetti
6. Scalable Isolation Of Mammalian Mitochondria For Nucleic Acid And Nucleoid Analysis
Ken-Wing Lee and Daniel F. Bogenhagen
7. Tracking Mitochondrial DNA In Situ
Anna Ligasova and Karel Koberna
PART II: Analysis of Mitochondrial DNA Replication, Mitochondrial DNA-Encoded Protein Translation and Mitochondrial DNA Copy Number
8. Analysis Of Replicating Mitochondrial DNA By In Organello Labeling And Two-Dimensional Agarose Gel Electrophoresis
Ian J. Holt, Lawrence Kazak, Aurelio Reyes, and Stuart R. Wood
9. Translation And Assembly Of Radiolabelled Mitochondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mitochondria
Luke E. Formosa, Annette Hofer, Christin Tischner, Tina Wenz, and Michael T. Ryan
10. Analysis Of Mitochondrial DNA Copy Number And Its Regulation Through DNA Methylation Of POLGA
Xin Sun, William Lee, Vijesh Vaghjiani, and Justin C. St. John
Part VI: Modification Of Mitochondrial
DNA
11. Engineered Mtzfns For Manipulation Of Human Mitochondrial DNA Heteroplasmy
Payam A. Gammage, Lindsey Van Haute, and Michal Minczuk
12. Generation Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice
Ian A. Trounce, Jessica Ackerley, and Matthew McKenzie
13. Import Of Fluorescent RNA Into Mitochondria Of Living Cells
Jaroslav Zelenka and Petr Jezek
Part V: Purification of Proteins Involved In Mitochondrial DNA Replication and Transcription
14. Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase
Fernando A. Rosado-Ruiz, Minyoung So, and Laurie S. Kaguni
15. Expression And Purification Of Mitochondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster
John P. Gajewski, Jamie J. Arnold, Tiina S. Salminen, Laurie S. Kaguni, and Craig E. Cameron
16. Purification And Comparative Assay Of Human Mitochondrial Single-Stranded DNA-Binding Protein
Grzegorz L. Ciesielski, Fernando A. Rosado-Ruiz, and Laurie S. Kaguni
17. Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mitochondrial DNA Replisome
Grzegorz L. Ciesielski, Vesa P. Hytoenen, and Laurie S. Kaguni
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2015
Pages: 205
Weight: 6788g
Availability: Available
Subcategories: Genetics
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