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Main description:
The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases. In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology. As a volume in the highly successful Methods in Molecular Biology (TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.
Contents:
1. Molecular Dermatology Comes of Age
Cristina Has and Cassian Sitaru
2. Molecular Diagnosis of Autoimmune Blistering Diseases
Daisuke Tsuruta, Teruki Dainichi, Takahiro Hamada, Norito Ishii, and Takashi Hashimoto
3. Molecular Diagnosis of Genodermatoses
Vesarat Wessagowit
4. Three-Dimensional Visualization of the Molecular Architecture of Cell-Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections
Ashraf Al-Amoudi and Achilleas S. Frangakis
5. 3D Visualization of Epidermal Langerhans Cells
Akiharu Kubo, Keisuke Nagao, and Masayuki Amagai
6. In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography
Viviane Mumprecht and Michael Detmar
7. Optical Imaging of HPV Infection in a Murine Model
Rhonda C. Kines, Hisataka Kobayashi, Peter L. Choyke, and Marcelino L. Bernardo
8. Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration
Hagar I. Labouta, Ulrich F. Schaefer, and Marc Schneider
9. Analysis of Cutaneous Somatic Mosaicism
Anna M.G. Pasmooij and Marcel F. Jonkman
10. Global Proteome Analyses of SILAC Labeled Skin Cells
Adrian Sprenger, Victoria Kuttner, Leena Bruckner-Tuderman, and Joern Dengjel
11. MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real Time PCR Method
Janosch Hildebrand, Adam Grundhoff, Stefan Gallinat, Horst Wenck, and Anja Knott
12. Cell Death in the Skin: How to Study Its Quality and Quantity?
Roman Makarov, Peter Geserick, Maria Feoktistova, and Martin Leverkus
13. In Vitro Pathogenicity Assay for Anti-Desmoglein Autoantibodies in Pemphigus
Ken Ishii and Masayuki Amagai
14. Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo
Mircea Teodor Chiriac
15. Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model
Jean Albrengues, Guerrino Meneguzzi, and Cedric Gaggioli
16. Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues
Alexander D. Boiko
17. Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors
Benjamin Toh, Alessandra Nardin, Xilei Dai, Jo Keeble, Valerie Chew, and Jean-Pierre Abastado
18. Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein "Kaede"-Transgenic Mice
Michio Tomura and Kenji Kabashima
19. Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach
Veronique J. Moulin
20. Bioengineered Skin Humanized Model of Psoriasis
Marta Carretero, Sara Guerrero-Aspizua, and Marcela Del Rio
21. Induction of Contact Hypersensitivity in the Mouse Model
Stefan F. Martin
22. Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells
Ganna Bilousova and Dennis R. Roop
23. RNAi-Mediated Gene Function Analysis in Skin
Slobodan Beronja and Elaine Fuchs
24. Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies
Wataru Nishie
25. Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII
Alina Sesarman and Cassian Sitaru
26. Animal Model for Cutaneous Leishmaniasis
Michael R. Fischer, Dominik John, Kordula Kautz-Neu, Anja Schermann, Kirsten Schwonberg, and Esther von Stebut
27. Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils and Macrophages
Dominik John, Michael R. Fischer, and Esther von Stebut
28. Zebrafish as a Model System to Study Heritable Skin Diseases
Qiaoli Li and Jouni Uitto
29. Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa
Alexander Nystroem, Leena Bruckner-Tuderman, and Johannes S. Kern
30. RNA Trans-Splicing for Genodermatoses
Johann W. Bauer, Eva M. Murauer, Verena Wally, and Ulrich Koller
Cristina Has and Cassian Sitaru
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: January, 2013
Pages: 610
Weight: 652g
Availability: Available
Subcategories: Dermatology, Endocrinology, General Issues
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