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MORE ABOUT THIS BOOK
Main description:
Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. Many of these techniques study potassium channels as cellular proteins as well as the resultant membrane biophysics. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters contain brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section, which highlights tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Potassium Channels: Methods and Protocols provides a wide range of approaches in order to aid current research projects and inspire new investigations.
Contents:
Part I: Methods for Studying Potassium Channel Transcription and mRNA Splicing
Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA-Protein Interactions by Chromatin Immunoprecipitation
Lezanne Ooi and Ian C. Wood
Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries
Alex Cheong, Samuel J. Fountain, and David J. Beech
Cloning of Potassium Channel Splice Variants from Tissues and Cells
Lie Chen and Michael J. Shipston
Part II: Methods for Studying the Cell Biology of Potassium Channel Proteins
Chemiluminescence Assays to Investigate Membrane Expression and Clathrin Mediated Endocytosis of KATP Channels
Andrew J. Smith and Asipu Sivaprasadarao
Investigation of KATP Channel Endocytosis by Immunofluorescence
Andrew J. Smith and Asipu Sivaprasadarao
Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting
Andrew J. Smith and Asipu Sivaprasadarao
Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts
Laura J. Sampson and Caroline Dart
Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein Lipid Overlay Assay
Alison M. Thomas and Andrew Tinker
Protein Complex Analysis of Native Brain Potassium Channels by Proteomics
Guillaume Sandoz and Florian Lesage
Part III: Electrophysiological Techniques for the Study of Potassium Channel Function
Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch Clamp Technique
Paolo Tammaro, Kenju Shimomura, and Peter Proks
Whole Cell Recording Using the Perforated Patch Clamp Technique
Jonathan D. Lippiat
Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration
Andrei I. Tarasov
Planar Patch Clamp: Advances in Electrophysiology
Andrea Bruggemann, Cecilia Farre, Claudia Haarmann, Ali Haythornthwaite, Mohamed Kreir, Sonja Stoelzle, Michael George, and Niels Fertig
Analyzing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers
Heidi de Wet, Jonathan D. Lippiat, and Marcus Allen
Part IV: Optical Techniques for the Study of Potassium Channel Function and Interactions
Using Bioluminescence Resonance Energy Transfer (BRET) to Measure Ion Channel Assembly
Gina M. Whitaker and Eric A. Accili
The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels
Adi Raveh, Inbal Riven, and Eitan Reuveny
The Voltage-Clamp Fluorometry Technique
Chris S. Ganhdi and Riccardo Olcese
Part V: Methods for Studying Potassium Channelopathies and Pharmacological Modulators
Identification of Mutations in the Kir6.2 Subunit of the KATP Channel
Sarah E. Flanagan and Sian Ellard
Modulation of Potassium Ion Channel Proteins Utilizing Antibodies
Mark L Dallas, Susan A. Deuchars, and Jim Deuchars
Fluorescence-Based Tl+-Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+-Activated
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 302
Weight: 772g
Availability: Available
Subcategories: Pharmacology
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