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MORE ABOUT THIS BOOK
Main description:
This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.
Contents:
Part I. Analysis of Heart Proteome
Two-dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics
Michael J. Dunn
Analyzing The Cardiac Muscle Proteome By Liquid Chromatography-Mass Spectrometry (Lc-ms) Based Expression Proteomics
Anthony O. Gramolini, Thomas Kislinger, Peter Liu, David H. Maclennan, and Andrew Emili
2-D Differential Gel Electrophoresis (DIGE) of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion
Jun Sakai, Hironori Ishikawa, Hideshi Satoh, Setsuko Yamamoto, Shinichi Kojima, and Masaharu Kanoaka
Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach
Alberto Lazaro, Julio Gallego-delgado, Julio Osende, Jesus Egido, and Fernando Vivanco
A Solubility Optimization Protocol for 2-D Gel Electrophoresis of Cardiac Tissue
Brian A. Stanley and Jennifer E. Van Eyk
A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium
Brian A. Stanley and Jennifer E. Van Eyk
Part II. Heart Subproteomes
Subcellular Fractionation
Mariana E. Guimaraes De Araujo and Alfons Huber
Subfractionation of Heart Tissue: The 'In Sequence' Myofilament Protein Extraction Of Myocardial Tissue
Lesley A. Kane, Irina Neverova, and Jennifer E. Van Eyk
Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and Seldi-tofT
Diane E. Bovenkamp, Brian A. Stanley and Jennifer E.van Eyk
Proteomic Analysis Of The Subunit Composition of Complex I (Nadh:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria
Ian M. Fearnley, Joe Carrol and John E. Walker
Identification of Targets of Phosphorylation in Heart Mitochondria
Roberta A. Gottliebie
Part III. Proteomics of Human Atheroma Plaques
Characterization of The Human Atheroma Plaque Secretome by Proteomic Analysis
Mari-CarmenDuran, Jose L. Martin-Ventura, Sebastian Mas, Maria G. Barderas, Veronica M. Darde, Ole N. Jensen, Jesus Egido, and Fernando Vivanco
Characterization of Hsp-27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome
Mari-Carmen Duran, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martin-Ventura, Jesus Egido, Fernando Vivanco, and Ole N. Jensen
Western Array Analysis of Human Atherosclerotic Plaque
Wim Martinetar
Part IV. The Proteome of Cells of The Cardiovascular System
The Proteome of Endothelial Cells
Jesus Gonzalez-Cabrero, Mayte Pozo, Mari-Carmen Duran, Rosario de Nicolas, Jesus Egido, and Fernando Vivanco
Proteomic Study of Caveolae and Rafts Isolated from Human Endothelial Cells
Richard R. Sprenger and Anton J. G. Horrevoets
Proteomic Identification of S-nitrosylated Proteins In Endothelial Cells
Antonio Martinez-Ruiz and Santiago Lamasgo
The Proteome and Secretome of Human Arterial Smooth Muscle Cell
Annabelle Dupont and Florence Pinet
Real-Time in Vivo Proteomic Identification Of Novel Kinase Substrates in Smooth Muscle
Anne A. Wooldridge and Timothy A. Haystead
Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain
Alexy V. Pshezhetsky
Isolation and Culture of Adult Mouse Cardiac Myocytes
Timothy D. OConnell, Manoj C. Rodrigo, and Paul C. Simpson
Proteomic Analysis Of Foam Cells
Peng-Yuang Yang, Yao-cheng Rui, Peng-Yuan Yang, and Yan-Ling Yuin
Isolation of The Platelet Releasate
Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire
Enrichment Of Phospho-Tyrosine Proteome Of Human Platelets By Immunoprecipitation
Martina Foy, Donald F. Harney, Kieran Wynne, and Patricia B. Maguire
Chapter 25
Veronica M. Darde, Mari-Carmen Duran, Jesus Egido, and Fernando Vivanco
Part V.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2010
Pages: 432
Weight: 635g
Availability: Available
Subcategories: Biochemistry
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