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Advanced Protocols in Oxidative Stress III
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Main description:

Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.


Contents:

Part I: Reactive Oxygen and Nitrogen Techniques

1. Derivatization and Detection of Small Aliphatic and Lipid-Bound Carbonylated Lipid Peroxidation Products by ESI-MS

Ivana Milic and Maria Fedorova

2. Specific Imaging and Tracking of Mitochondria in Live Cells by a Photostable AIE Luminogen

Chris W.T. Leung, Yuning Hong, and Ben Zhong Tang

3. Analysis of Relationship Between Oxidized Phospholipid Structure and Interaction with the Class B Scavenger Receptors

Detao Gao, Larry W. Sayre, and Eugene A. Podrez

4. A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA

Takayuki Shibamoto

5. Nitric Oxide Availability as a Marker of Oxidative Stress

Dan Pierini and Nathan S. Bryan

6. Quantification of DNA Repair Capacity Towards Oxidatively Damaged DNA in Subcellular and Cellular Systems by a Non-Radioactive Cleavage Assay

Ingrit Hamann and Andrea Hartwig

7. Chiral Phase-HPLC Separation of Hydroperoxyoctadecenoic Acids and Their Biosynthesis by Fatty Acid Dioxygenases

Ernst H. Oliw and Anneli Wennman

8. Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and H2S Repair

Zhangrong Lou, Peng Li, and Keli Han

9. Evaluation of Physical Integrity of Lipid Bilayer Under Oxidative Stress: Application of Fluorescence Microscopy and Digital Image Processing

Ran Liang, Jian-Ping Zhang, and Leif H. Skibsted

10. Electrochemical Detection of Glutathione S-Transferase: An Important Enzyme in the Cell Protective Mechanism Against Oxidative Stress

Manuel C. Martos-Maldonado, Juan M. Casas-Solvas, Antonio Vargas-Berenguel, and Luis Garcia-Fuentes

11. Using Nanosensors for In Situ Monitoring and Measurement of Nitric Oxide and Peroxynitrite in a Single Cell

Tadeusz Malinski

Part II: Antioxidant Technology and Application

12. Curcuma longa Attenuates Carbon Tetrachloride-Induced Oxidative Stress in T Lymphocyte Subpopulations

H.A. Abu-Rizq, Mohamed H. Mansour, and Mohammad Afzal

13. Prolongation of the Lag Time Preceding Peroxidation of Serum Lipids: A Measure of Antioxidant Capacity

Ilya Pinchuk and Dov Lichtenberg

14. Identification of (Anti-Oxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements

Eric Deconinck, Deborah Custers, and Jacques Omer De Beer

15. Simultaneous Determination of Ascorbic Acid, Aminothiols, and Methionine in Biological Matrices Using Ion-Pairing RP-HPLC Coupled with Electrochemical Detector

Muhammad Imran Khan, Zafar Iqbal, and Abad Khan

16. Portable Nanoparticle Based Sensors for Antioxidant Analysis

Erica Sharpe and Silvana Andreescu

17. Fe3+-Fe2+ Transformation Method: An Important Antioxidant Assay

Ilhami Gulcin

18. Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity

Augustin C. Mot, Cristina Bischin, Grigore Damian, and Radu Silaghi-Dumitrescu

19. Computational Studies on Conformation, Electron Density Distributions, and Antioxidant Properties of Anthocyanidins

Ricardo A. Mosquera, Laura Estevez, and Mercedes Garcia-Bugarin

20. Automatic Flow Injection Analysis (FIA) Determination of Total Reducing Capacity in Serum and Urine Samples

Marcela A. Segundo, Ildiko V. Toth, Luis M. Magalhaes, and Salette Reis

21. Mass Spectrometry Detection of Isolevuglandin Adduction to Specific Protein Residues

Casey D. Charvet and Irina A. Pikuleva

22. Preoxiredoxin Family Members (Prx3 & Prx4) and Pregnancy Disorder (Recurrent Pregnancy Loss)

Behrouz Gharesi-Fard

23. Substituted Pyridoindoles as Biological Antioxidants: Drug Design, Chemical Synthesis, and Biological Activity

Lucia Kovacikova, Magdalena Majekova, and Milan Stefek

24. Measuring Antioxidant and Prooxidant Capacity Using the Crocin Bleaching Assay (CBA)

Stella A. Ordoudi and Maria Z. Tsimidou

25. Simultaneous Determination of the Endogenous Free Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP HPLC Coupled with Electrochemical Detector

Muhammad Imran Khan, Zafar Iqbal, and Abad Khan

26. Aryl-Acetic and Cinnamic Acids as Lipoxygenase Inhibitors with Antioxidant, Anti-Inflammatory, and Anti-Cancer Activity

Dimitra Hadjipavlou-Litina and Eleni Pontiki

27. Liquid Chromatography and Mass Spectrometry for the Analysis of N- -Methylamino-L-Alanine with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate

W. Broc Glover, Steven A. Cohen, and Susan J. Murch

Part III: Gene Expression

28. Intracellular Distribution of Glutathionylated Proteins in Cultured Dermal Fibroblasts by Immunofluorescence

Stefania Petrini, Valentina D'Oria, and Fiorella Piemonte

29. New Analytical Strategies Applied to the Determination of Coenzyme Q10 in Biological Matrix

Contin Mario, Flor Sabrina, Martinefski Manuela, Lucangioli Silvia, and Tripodi Valeria

30. Binding and Cytotoxic Trafficking of Cholesterol Hydroperoxides by Sterol Carrier Protein-2

Albert W. Girotti and Tamas Kriska

Part IV: Biostatistics

31. A Toolkit for Clinical Statisticians to Fix Problems Based on Biomarker Measurements Subject to Instrumental Limitations: From Repeated Measurement Techniques to a Hybrid Pooled-Unpooled Design

Albert Vexler, Ge Tao, and Xiwei Chen


PRODUCT DETAILS

ISBN-13: 9781493914401
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2014
Pages: 435
Weight: 652g
Availability: Available
Subcategories: Biochemistry
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