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Hox Genes
Methods and Protocols
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Main description:

Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences. This detailed volume begins with a section on discovery and functional analysis of Hox genes and then it continues onward to discuss mode of action and biomedical applications of Hox proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Expert and practical, Hox Genes: Methods and Protocols serves as an ideal guide to researchers striving to move forward in this dynamic and exciting area of study.


Contents:

Part I: Hox Genes: From Discovery to Functional Analysis

1. Discovery and Classification of Homeobox Genes in Animal Genomes

Ferdinand Marletaz, Jordi Paps, Ignacio Maeso, and Peter W.H. Holland

2. How to Study Hox Gene Expression and Function in Mammalian Oocytes and Early Embryos

Delphine Paul, Caroline Sauvegarde, Rene Rezsohazy, and Isabelle Donnay

3. Genetic Lineage Tracing Analysis of Anterior Hox Expressing Cells

Brigitte Laforest, Nicolas Bertrand, and Stephane Zaffran

4. A Genetic Strategy to Obtain P-Gal4 Elements in the Drosophila Hox Genes

Luis de Navas, David Foronda, Delia del Saz, and Ernesto Sanchez-Herrero

5. Hox Complex Analysis Through BAC Recombineering

Mark Parrish, Youngwook Ahn, Christof Nolte, Bony De Kumar, and Robb Krumlauf

6. The Genetics of Murine Hox Loci: Tamere, String, and Panthere to Engineer Chromosome Variants

Patrick Tschopp and Denis Duboule

7. Topological Organization of Drosophila Hox Genes Using DNA Fluorescent In Situ Hybridization

Frederic Bantignies and Giacomo Cavalli

8. Mining the Cis-Regulatory Elements of Hox Clusters

Navneet Kaur Matharu and Rakesh K. Mishra

9. Functional Analysis of Hox Genes in Zebrafish

Franck Ladam and Charles G. Sagerstroem

10. Transgenesis in Non-Model Organisms: The Case of Parhyale

Zacharias Kontarakis and Anastasios Pavlopoulos

11. Tissue Specific RNA Isolation in Drosophila Embryos: A Strategy to Analyze Context Dependent Transcriptome Landscapes Using FACS

Arnaud Defaye and Laurent Perrin

12. Hox Transcriptomics in Drosophila Embryos

Maria Polychronidou and Ingrid Lohmann

Part II: Hox Proteins: Mode of Action and Biomedical Applications

13. Measuring Hox-DNA Binding by Electrophoretic Mobility Shift Analysis

Kelly Churion, Ying Liu, Hao-Ching Hsiao, Kathleen S. Matthews, and Sarah E. Bondos

14. Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue

Shilu Amin and Nicoletta Bobola

15. ChIP for Hox Proteins from Drosophila Imaginal Discs

Pavan Agrawal and L.S. Shashidhara

16. SELEX-seq: A Method for Characterizing the Complete Repertoire of Binding Site Preferences for Transcription Factor Complexes

Todd R. Riley, Matthew Slattery, Namiko Abe, Chaitanya Rastogi, Richard Mann, and Harmen Bussemaker

17. DamID as an Approach to Studying Long Distance Chromatin Interactions

Fabienne Cleard, Francois Karch, and Robert K. Maeda

18. cgChIP: A Cell Type and Gene Specific Method for Chromatin Analysis

Marios Agelopoulos, Daniel J. McKay, and Richard S. Mann

19. Bimolecular Fluorescence Complementation (BiFC) in Live Drosophila Embryos

Marilyne Duffraisse, Bruno Hudry, and Samir Merabet

20. Hox Protein Interactions: Screening and Network Building

Isabelle Bergiers, Barbara Lambert, Sarah Daakour, Jean-Claude Twizere, and Rene Rezsohazy

21. Rational Drug Repurposing Using sscMap Analysis in a HOX-TALE Model of Leukemia

Laura M.J. Kettyle, Fabio G. Liberante, and Alexander Thompson


PRODUCT DETAILS

ISBN-13: 9781493912414
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2014
Pages: 360
Weight: 9663g
Availability: Available
Subcategories: General Issues, Genetics
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