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MORE ABOUT THIS BOOK
Main description:
The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.
Feature:
Approaches the study of heat shock transcription factors both in vivo and in vitro
Features step-by-step protocols which are tested and ready for the lab
Includes expert tips and vital implementation advice to ensure successful results
Back cover:
The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.
Contents:
1. Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi
2. The Role Of Heat Shock Factors in Stress-Induced Transcription
Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood
3. Hsp90 and Client Protein Maturation
Natalie Wayne, Parul Mishra, and Daniel N. Bolon
4. The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function
Marc B. Cox and Jill L. Johnson
5. Detecting Hsp90 Phosphorylation
Mehdi Mollapour and Len Neckers
6. Role of Molecular Chaperones in Biogenesis of the Protein Kinome
Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, and Avrom J. Caplan
7. Nucleotide Exchange Factors for Hsp70 Chaperones
Heike Rampelt, Matthias P. Mayer, and Bernd Bukau
8. Reconstitution of CHIP E3 Ubiquitin Ligase Activity
Hong Yu Ren, Cam Patterson, Douglas M. Cyr, and Meredith F.N. Rosser
9. Structure-Functions of HspB1 (Hsp27)
André-Patrick Arrigo
10. Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene
Punit Kaur, Ganachari M. Nagaraja, Khalid Bin Abdulrahman, and Alexzander Asea
11. Quantification of HSP27 and HSP70 Molecular Chaperone Activities
Jessica Gobbo, Caroline Gaucher-Di-Stasio, Stéphanie Weidmann, Jean Guzzo, and Carmen Garrido
12. Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA
H. Elyse Ireland and John H.H. Williams
13. Analysis of Heat Shock Protein Localization Using Flow Cytometry
Nina C. Dempsey, Francesca Leoni, Christine Hoyle, and John. H.H. Williams
14. Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry
Punit Kaur, Khalid Bin Abdulrahman, and Alexzander Asea
15. Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks
Yunchen Gong, Zhaolei Zhang, and Walid A. Houry
16. Hsp70: Anti-Apoptotic and Tumorigenic Protein
Anne-Laure Rérole, Gaëtan Jego, and Carmen Garrido
17. Determination of Cell Survival or Death
Alexander E. Kabakov, Vladimir A. Kudryavtsev, and Vladimir L. Gabai
18. Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, and Alberto J.L. Macario
19. Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells
Desheng Weng, Stuart K. Calderwood, and Jianlin Gong
20. Isolation of Heat Shock Protein Complexes
Fernando D. Cuello-Carrión, Mariel A. Fanelli, Niubys Cayado-Gutiérrez, Gisela Castro, and Daniel R. Ciocca
21. Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein
Xiang-Yang Wang, Huanfa Yi, Xiaofei Yu, Damin Zuo, and John R. Subjeck
22. Investigating Receptors for Extracellular Heat Shock Proteins
Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood
23. Analysis of Cellular Migration Using a Two-Chamber Methodology
Michelle M. Kouspou and John T. Price
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: September, 2011
Pages: 320
Weight: 833g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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CUSTOMER REVIEWS
From the reviews:
“This book details current methods and protocols essential for understanding the biological properties and roles of the molecular chaperones … . It appears to be written for all scientists interested in understanding the roles of molecular chaperones, which would include students and researchers interested in heat shock proteins. … This will be a useful guide for all scientists interested in the field of heat shock proteins and molecular chaperones and their role in health and disease.” (Omer Iqbal, Doody’s Book Reviews, December, 2011)