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Main description:
With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
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Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.Feature:
-Features methods and protocols relevant for both fixed and live cells from everything from bacteria to mammalian models
-Provides detailed techniques from established and up-and-coming experts in the field
-Includes vital notes on troubleshooting and avoiding known pitfalls for easier implementation of the methods
Back cover:
With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
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Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.Contents:
Part I: Visualizing mRNAs In situ
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1. Single Molecule Imaging of RNA In situ
           Mona Batish, Arjun Raj and Sanjay Tyagi
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2. FISH and Immunofluorescence Staining in Chlamydomonas
           James Uniacke, Daniel Colón-Ramos, and William Zerges
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3. High Resolution Fluorescent In situ Hybridization in Drosophila
           Eric Lécuyer
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4. Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In situ Hybridization
           Alexandre Paix, Janet Chenevert, and Christian Sardet
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5. Visualization of mRNA Localization in Xenopus Oocytes
           James A. Gagnon and Kimberly L. Mowry
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6. Visualization of mRNA Expression in the Zebrafish Embryo
           Yossy Machluf and Gil Levkowitz
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7. High-Resolution Fluorescence In situ Hybridization to Detect mRNAs in Neuronal Compartments In vitro and In vivo
           Sharon A. Swanger, Gary J. Bassell, and Christina Gross
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8. Localization of mRNA in Vertebrate Axons Using In situ Hybridization
           José Roberto Sotelo-Silveira, Aldo Calliari, Alejandra Kun, Victoria Elizondo, LucÃa Canclini, and José Roberto Sotelo
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Part II: Visualizing mRNAs In vivo Using Molecular Probes or Reconstituted Fluorescent Proteins
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9. Tiny Molecular Beacons for In vivo mRNA Detection
           Diana P. Bratu, Irina E. Catrina, and Salvatore A.E. Marras
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10. Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA
           Antony K. Chen, Won Jong Rhee, Gang Bao, and Andrew Tsourkas
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11. Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells
           Takeaki Ozawa and Yoshio Umezawa
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12. Visualization of Induced RNA in Single Bacterial Cells
           Azra Borogovac and Natalia E. Broude
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Part III: Visualizing mRNAs In vivo Using Aptamers and Intact Fluorescent Proteins
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13. Visualizing mRNAs in Fixed and Living Yeast Cells
           Franck Gallardo and Pascal Chartrand
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14. In vivo Visualization of RNA Using the U1A-Based Tagged RNA System
           Sunglan Chung and Peter A. Takizawa
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15. Visualizing Endogenous mRNAs in Living Yeast Using m-TAG, a PCR-Based RNA Aptamer Integration Method, and Fluorescence Microscopy
           Liora Haim-Vilmovsky and Jeffrey. E. Gerst
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16. Imaging mRNAs in Living Mammalian Cells
           Sharon Yunger and Yaron Shav-Tal
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17. Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport during Drosophila Oogenesis
           Katsiaryna Belaya and Daniel St. Johnston
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Part IV: Use of Cell Fractionation to Demonstrate the Sub-Cellular Localization of RNA
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18. Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria
           Erez Eliyahu, Daniel Melamed, and Yoav Arava
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19. Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation
           Sujatha Jagannathan, Christine Nwosu, and Christopher V. Nicchitta
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20. Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and Affinity Purification Procedure
           Gadi Zipor, Cecile Brocard, and Jeffrey. E. Gerst
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21. Profiling Axonal mRNA Transport
           Dianna E. Willis and Jeffery L. Twiss
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22. RNA Purification from Tumor Cell Protrusions Using Porous Polycarbonate Filters
           Jay Shankar and Ivan R. Nabi
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Part V: Affinity Purification of mRNAs and the Identification of Trans-Acting Factors
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23. RNA-Binding Protein Immunopurification-Microarray (RIP-Chip) Analysis to Profile Localized RNAs
           Alessia Galgano and André P. Gerber
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24. RaPID: An Aptamer-Based mRNA Affinity Purification Technique for the Identification of RNA and Protein Factors Present in Ribonucleoprotein Complexes
           Boris Slobodin and Jeffrey E. Gerst
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25. RIP: An mRNA Localization Technique
           Sabarinath Jayaseelan, Francis Doyle, Salvatore Currenti, and Scott Tenenbaum
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26. The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and RNA-Protein Complexes
           Scott C. Walker, Paul D. Good, Theresa A. Gipson, and David R. Engelke
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Part VI: Use of Bioinformatics to Identify Cis-Acting Motifs and Structures in RNAs
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27. Identifying and Searching for Conserved RNA Localization Signals
           Russell S. Hamilton and Ilan Davis
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28. Computational Prediction of RNA Structural Motifs Involved in Post Transcriptional Regulatory Processes
           Michal Rabani, Michael Kertesz, and Eran Segal
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: April, 2011
Pages: 465
Weight: 1145g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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