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Main description:
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that "the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer" [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.
Contents:
1. Utilization of Liposomes for Studying Drug Transfer and Uptake
Alfred Fahr and Xiangli Liu
2. The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer Vesicles
James R. Reed
3. Use of Liposomes to Study Cellular Osmosensors
Reinhard Kramer, Sascha Nicklisch, and Vera Ott
4. Studying Mechanosensitive Ion Channels Using Liposomes
Boris Martinac, Paul R. Rohde, Andrew R. Battle, Evgeny Petrov, Prithwish Pal, Alexander Fook Weng Foo, Valeria Vasquez, Thuan Huynh, and Anna Kloda
5. Studying Amino Acid Transport Using Liposomes
Cesare Indiveri
6. Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes
Chris Hoefer, Andreas Herrmann, and Peter Muller
7. Liposomal Reconstitution of Monotopic Integral Membrane Proteins
Zahra MirAfzali and David L. DeWitt
8. The Reconstitution of Actin Polymerization on Liposomes
Mark Stamnes and Weidong Xu
9. Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains Under Physiological Ionic-Strength Conditions
L.-Ruth Montes, Hasna Ahyayauch, Maitane Ibarguren, Jesus Sot, Alicia Alonso, Luis A. Bagatolli, and Felix M. Goni
10. Visualization of Lipid Domain Specific Protein Sorting in Giant Unilamellar Vesicles
Martin Stoeckl, Joerg Nikolaus, and Andreas Herrmann
11. Biosynthesis of Proteins Inside Liposomes
Pasquale Stano, Yutetsu Kuruma, Tereza Pereira de Souza, and Pier Luigi Luisi
12. Study of Respiratory Cytochromes in Liposomes
Iseli L. Nantes, Cintia Kawai, Felipe S. Pessoto, and Katia C.U. Mugnol
13. Use of Liposomes to Evaluate the Role of Membrane Interactions onAntioxidant Activity
Salette Reis, Marlene Lucio, Marcela Segundo, and Jose L.F.C. Lima
14. Studying Colloidal Aggregation Using Liposomes
Juan Sabin, Gerardo Prieto, Juan M. Ruso, and Felix Sarmiento
15. Assessment of Liposome-Cell Interactions
Jan A.A.M. Kamps
16. Methods to Monitor Liposome Fusion, Permeability, and Interaction with Cells
Nejat Duzgunes, Henrique Faneca, and Maria C. Pedroso de Lima
17. The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes
David Miller and Paula J. Booth
18. Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling
Witold K. Subczynski, Marija Raguz, and Justyna Widomska
19. Membrane Translocation Assayed by Fluorescence Spectroscopy
Jana Broecker and Sandro Keller
20. Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance Spectroscopy
Hugo Ruben Arias
21. Environmental Scanning Electron Microscope Imaging of Vesicle Systems
Yvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U.R. Mohammed, Sarah E. McNeil, and Anil Vangala
22. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Brigitte Papahadjopoulos-Sternberg
23. Atomic Force Microscopy for the Characterization of Proteoliposomes
Johannes Sitterberg, Maria Manuela Gaspar, Carsten Ehrhardt, and Udo Bakowsky
24. Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID
Sophia Hatziantoniou and Costas Demetzos
25. Viscometric Analysis of DNA-Lipid Complexes
Sadao Hirota and Nejat Duzgunes
26. Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Edwin
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2009
Pages: 564
Weight: 1373g
Availability: Available
Subcategories: Pharmacology
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