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Main description:
In RNA Mapping- Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study RNA. These include protocols for the consequence of the emerging interest in the characterization of cellular RNAs urged by their potential use as diagnostic biomarkers or therapeutic targets. In particular, the biological relevance of microRNAs in human physiology and disease development is highlighted in the 16 chapters focused on methods for their physical and functional mapping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Mapping- Methods and Protocols provides instruction and inspiration for scientists who are facing the challenges of the discovery and/or functional characterization of RNA molecules for a wide variety of applications ranging from novel biomedical diagnostics to therapeutics and biomaterials.
Contents:
Part I-Structural RNA Mapping
1. Full-Length Characterization Of Transcribed Genomic Regions
Marc Rene Reboll, M. Lucrecia Alvarez, and Mahtab Nourbakhsh
2. Rapid Mapping Of RNA 3and 5Ends
Victoria Zismann and Mahtab Nourbakhsh
3. Single Nucleotide Mapping Of RNA 5and 3Ends
Mahtab Nourbakhsh
4. RNA Secondary Structure Analysis
Mahtab Nourbakhsh
5. Tertiary Structure Mapping Of Pri-Mirna17~92
Steven G. Chaulk and Richard P. Fahlman
6. In Situ Hybridization Detection Of Micrornas Using LNA Oligonucleotides
Olga Beltcheva and Stefania Cotta Done
7. Quantification Of Micrornas By A Simple And Specific Qpcr Method
Peter Busk and Susanna Cirera
8. RNA Isolation For Small RNA Next Generation Sequencing From Acellular Biofluids
Kasandra L. Burgos and Kendall Van Keuren-Jensen
9. Sequencing Small RNA: Introduction and Data Analysis Fundamentals
Jai Prakash Mehta
10. Measuring Expression Levels Of Small Regulatory RNA Molecules From Formalin-Fixed, Paraffin-Embedded Samples and Body Fluids
Adrienn Gyongyosi, Otto Docs, Zsolt Czimmerer, Laszlo Orosz, Attila Horvath, Olga Toeroek, Gabor Mehes, Laszlo Nagy, and Balint L Balint
11. Microrna Profiling In Plasma Or Serum Using Quantitative RT-Qpcr
Marina C. Costa, Ana Lucia Leitao, and Francisco J. Enguita
12. Microrna Profiling Of Exosomes Isolated From Biofluids And Conditioned Media
Sweta Rani
13. Isolation Of Urinary Exosomes For RNA Biomarker Discovery Using A Simple, Fast, And Highly Scalable Method
M. Lucrecia Alvarez
Part II- Functional RNA Mapping
14. Identification Of Actively Translated Mrnas
Marc Rene Reboll and Mahtab Nourbakhsh
15. Mapping Of Internal Ribosome Entry Sites (IRES)
Sarah Mehrtens and Marc Rene Reboll
16. Mapping Of Protein Binding RNA Elements
Marc Rene Reboll
17. Purification Of RNA Binding Proteins
Birgit Ritter and Marc Rene Reboll
18. De Novo Approach To Classify Protein-Coding And Non-Coding Transcripts By Utilizing Sequence Intrinsic Composition
Haitao Luo, Dechao Bu, Liang Sun, and Yi Zhao
19. Computational Methods To Predict Long Non-Coding RNA Functions Based On Co-Expression Network
Yi Zhao, Haitao Luo, Xiaowei Chen, Yi Xiao, and Runsheng Chen
20. Microrna Biogenesis - Dicing Assay
Carlos A. Melo and Sonia A. Melo
21. Faster Experimental Validation Of Microrna Targets Using Cold Fusion Cloning And A Dual Firefly-Renilla Luciferase Reporter Assay
M. Lucrecia Alvarez
22. Experimental Validation Of Predicted Mammalian Micrornas Of Mirtron Origin
Anita Schamberger and Tamas I. Orban
23. A Guide For Microrna Target Prediction And Analysis Using Web-Based Applications
Ana Lucia Leitao, Marina C. Costa, and Francisco J. Enguita
24. Tapping Microrna Regulation Networks Through Integrated Analysis Of Microrna-Mrna High-Throughput Profiles
Anthony D. Saleh and Hui Cheng
25. Mirwalk Database For Microrna-Target Interactions
Harsh Dweep, Norbert Gretz And Carsten Sticht
26. A Schematic Workflow For Collecting The Interaction Information Between Copy Number Variants And Micrornas Using Existing Resources
Harsh Dweep, Norbert Gretz, and Kyriakos Felekkis
27. SYBR Green and Taqman PCR Arrays: Expression Profile Of Genes Relevant To A Pathway Or Disease State
M. Lucrecia Alvarez And Stefania Cotta Done
28. Comprehensive Meta-Analysis Of Microrna Expression Using A Robust Rank Aggregation Approach
Urmo Vosa, Raivo Kolde, Jaak Vilo, Andres Metspalu, and Tarmo Annilo
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: July, 2014
Pages: 335
Weight: 9582g
Availability: Available
Subcategories: Genetics
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