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MORE ABOUT THIS BOOK
Main description:
This book presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. The concluding section of the book highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.
Contents:
Part I. Cardiac Gene Expression Profiling; The Global Perspective
Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells
Yurong Liang, Xin Lu, and David L. Perkins
Expression Profiling Using Affymetrix GeneChip' Probe Arrays
Martina Schinke-Braun, and Jennifer A. Couget
Serial Analysis of Gene Expression (SAGE): A Useful Tool to Analyze the Cardiac Transcriptome
Kirill V. Tarasov, Sheryl A. Brugh, Yelena S. Tarasova, and Kenneth R. Boheler
Functional Genomics by cDNA Subtractive Hybridization
Christophe Depre
Statistical Methods in Cardiac Gene Expression Profiling: From Image to Function
Sek Won Kong
Part II. Cardiac Gene Regulation-Gene-Specific mRNA Measurement in the Myocardium
Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Nicola King
Quantitative (Real-Time) RT-PCR in Cardiovascular Research
Kevin John Ashton and John Patrick Headrick
RNase Protection Assay for Quantifying Gene Expression Levels
Yongxia Qu and Mohamed Boutjdir
In Situ Hybridization: A Technique to Study Localization of Cardiac Gene Expression
Thierry P. Calmels and David Mazurais
Part III. Cardiac Gene Regulation-Promoter Characterization in the Myocardium
Characterization of Cis-Regulatory Elements and Transcription Factor Binding: Gel Mobility Shift Assay
Jim Jung-Ching Lin, Shaun E. Grosskurth, Shannon M. Jaacks, Elisabeth A. Gustafson-Wagner, and Qin Wang
Mapping Transcriptional Start Sites and in silico DNA Footprinting
Martin E Cullen and Paul JR Barton
Characterization of Cardiac Gene Promoter Activity: Reporter Constructs and Heterologous Promoter Studies
Hsiao-Huei Chen and Alexandre F. R. Stewart
Part IV. In Silico Assessment of Regulatory Cis-Elements and GeneRegulation
Comparative Genomics: A Tool to Functionally Annotate Human DNA
Jan-Fang Cheng, James R. Priest, and Len A. Pennacchio
Developing Computational Resources in Cardiac Gene Expression
Michael B. Bober and Raimond Winslow
Part V. Cardiac Single Nucleotide Polymorphisms
In Silico Analysis of SNPs and Other High Throughput Data
Neema Jamshidi, Thuy D. Vo, and Bernhard O. Palsson
Discovery and Identification of Sequence Polymorphisms and Mutations With MALDI-TOF Ms
Dirk van den Boom and Mathias Ehrich
Part VI. Gene Overexpression and Targeting in the Myocardium
Conditional Targeting: Inducible Deletion by Cre Recombinase
Kelly R. O'Neal and Ramtin Agah
Cardiomyocyte Preparation, Culture, and Gene Transfer
Alexander H. Maass and Massimo Buvoli
Adeno-Associated Viral Vector Delivered Hypoxia-Inducible Gene Expression in Ischemic Hearts
Hua Su and Yuet Wai Kan
Virus-Mediated Gene Expression-Lentivisus
Jing Zhao and Andrew ML Lever
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: March, 2007
Pages: 378
Weight: 804g
Availability: Available
Subcategories: Biochemistry, Cardiovascular Medicine, Genetics
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