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MORE ABOUT THIS BOOK
Main description:
Driving further the research on mammalian alkaline phosphatase structure and function, Phosphatase Modulators collects expert contributions into one "how to" manual for basic scientists interested in initiating a drug discovery effort. While this book contains the traditional method chapters and some typical reviews on the structure and known functions of phosphatases, other contributions are meant to discuss approaches and alternatives useful in making "go/no-go" decisions in high throughput screening (HTS) and lead optimization campaigns. Many chapters focus on tissue-nonspecific alkaline phosphatase (TNAP) as well as protein phosphatases. Written for the highly successful Methods in Molecular Biology series, chapters in this volume include the kind of detail and key implementation advice that promotes reproducible results.
Step-by-step and practical, Phosphatase Modulators offers a path to understanding many of the facets and complexities associated with undertaking a drug discovery effort and will serve as a roadmap to initiating those efforts.
Contents:
1. Chemical Probe Development Versus Drug Development
Michael R. Jackson
2. Phosphatase High-Throughput Screening Assay Design and Selection
Eduard A. Sergienko
3. Multisystemic Functions of Alkaline Phosphatases
Rene Buchet, Jose Luis Millan, and David Magne
4. Robotic Implementation of Assays: Tissue-Nonspecific Alkaline Phosphatase (TNAP) Case Study
Thomas D.Y. Chung
5. Inhibitors of Tissue-Nonspecific Alkaline Phosphatase (TNAP): From Hits to Leads
Peter Teriete, Anthony B. Pinkerton, and Nicholas D.P. Cosford
6. A Method for Direct Assessment of Tissue-Nonspecific Alkaline Phosphatase (TNAP) Inhibitors in Blood Samples
Eduard Sergienko, Qing Sun, and Chen-Ting Ma
7. Isolation and Characteristics of Matrix Vesicles
Rene Buchet, Slawomir Pikula, David Magne, and Saida Mebarek
8. The Use of Tissue-Nonspecific Alkaline Phosphatase (TNAP) and PHOSPHO1 Inhibitors to Affect Mineralization by Cultured Cells
Tina Kiffer-Moreira and Sonoko Narisawa
9. Modulators of Intestinal Alkaline Phosphatase
Ekaterina V. Bobkova, Tina Kiffer-Moreira, and Eduard A. Sergienko
10. New Activity Assays for ENPP1 with Physiological Substrates ATP and ADP
Chen-Ting Ma and Eduard Sergienko
11. Structure of Acid Phosphatases
Cesar L. Araujo and Pirkko T. Vihko
12. Purification of Prostatic Acid Phosphatase (PAP) for Structural and Functional Studies
Annakaisa M. Herrala, Ileana B. Quintero, and Pirkko T. Vihko
13. Protein Tyrosine Phosphatases: Structure, Function, and Implication in Human Disease
Lutz Tautz, David A. Critton, and Stefan Grotegut
14. High-Throughput Screening for Protein Tyrosine Phosphatase Activity Modulators
Lutz Tautz and Eduard A. Sergienko
15. Evaluating Effects of Tyrosine Phosphatase Inhibitors on T Cell Receptor Signaling
Souad Rahmouni, Laurence Delacroix, Wallace H. Liu, and Lutz Tautz
16. Interactor-Guided Dephosphorylation by Protein Phosphatase-1
Shannah Boens, Kathelijne Szeker, Aleyde Van Eynde, and Mathieu Bollen
17. Structure, Regulation, and Pharmacological Modulation of PP2A Phosphatases
Caroline Lambrecht, Dorien Haesen, Ward Sents, Elitsa Ivanova, and Veerle Janssens
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: July, 2013
Pages: 310
Weight: 7611g
Availability: Available
Subcategories: Biochemistry, Pharmacology
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