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MORE ABOUT THIS BOOK
Main description:
Heart disease is the leading cause of death in developed countries. Recent experimental advances featuring cellular, molecular, and genetic tools and technologies offer the potential for new therapeutic strategies directed toward remediation of inherited and acquired heart diseases. Whether these recent basic science advances will ultimately translate to clinical efficacy for patients with heart disease is unknown and is important to ascertain. Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications is designed to provide the reader with up-to-date coverage of a myriad of specific methodo- gies and protocols for gene and cell transfer to the myocardium. Each chapter features a "Notes" section that provides useful "how to" problem-solving insights that are often left unstated in standard published protocols. Cardiac Cell and Gene Transfer: Principles, Protocols, and Appli- tions addresses principles and applications of cell and gene transfer to the heart, including protocols for vector production and purification. Detailed step-by-step methods and applications for first/second-generation adenoviral vectors, adeno-associated vectors, gutted adenoviral vectors, and lentiviral vectors are included. Additionally, detailed methods for cardiac cell grafting and transplantation are provided, and these chapters highlight the prospects of cell-based therapies for cardiac repair. The book also covers specific in vivo techniques for cardiac gene transfer, and specifies subsequent cellular and organ-level physiological assessment techniques and protocols. Accordingly, this book is designed for basic science and clinical researchers in the academic, pharmaceutical, and biotechnology sectors of the cardiovascular community.
Contents:
Part I. Vectors for Cardiac Gene Transfer: Production, Purification, and Applications
Adenoviral Vectors: Production and Purification
Faris P. Albayya and Joseph M. Metzger
Gutted Adenoviral Vectors for Gene Transfer to Muscle
Jeannine M. Scott and Jeffrey S. Chamberlain
Dual Vector Expansion of the Recombinant AAV Packaging Capacity
Dongsheng Duan, Yongping Yue, and John F. Engelhardt
Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes
Tsuyoshi Sakoda, Noriyuki Kasahara, and Larry Kedes
Part II. Cell Transfer to Myocardium: Protocols and Applications
Cell Therapy in the Heart: Cell Production, Transplantation, and Applications
Kevin S. Cahill, Catalin Toma, Mark F. Pittenger, Paul D. Kessler, and Barry J. Byrne
Cardiac Cell Transplantation: Protocols and Applications
Steven M. White and William C. Claycomb
Cell Grafting for Cardiac Repair
Hans Reinecke and Charles E. Murry
Part III. Vectors for Cardiac Gene Transfer: In Vitro and In Vivo Assays and Approaches
Suppressor tRNAs: Protocols and Applications for Cardiac Gene Transfer
Massimo Buvoli, Ada Buvoli, and Leslie A. Leinwand
Antisense Oligonucleotides: Design, Construction, and Applications to Cardiac Allograft Transfer
Leora B. Balsam, Douglas N. Miniati, and Robert C. Robbins
Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants
Guanyi Lu and D. Keith Bishop
Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes
Jennifer C. Hirsch, Andrea R. Borton, and Joseph M. Metzger
Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes
Margaret V. Westfall
Part IV. In Vivo Cardiac Gene Transfer: Protocols, Applications, and Physiological Assessment
Embryonic and Neonatal Cardiac Gene Transfer In Vivo
Geir Christensen, Peter J. Gruber, Yibin Wang, and Kenneth R. Chien
Efficient Viral Gene Transfer to Rodent Hearts In Vivo
Federica del Monte and Roger J. Hajjar
Direct Gene Transfer to the Adult Rodent Myocardium In Vivo
Michael L. Szatkowski, Margaret V. Westfall, and Joseph M. Metzger
Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures
Damien Logeart and Jean-Jacques Mercadier
Coronary Perfusion Cocktails for In Vivo Gene Transfer
Stephan E. Lehnart and J. Kevin Donahue
Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of b-Adrenergic Receptor Signaling Components
Hendrik T. Tevaearai and Walter J. Koch
Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo
Dimitrios Georgakopoulos and David A. Kass
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 266
Weight: 398g
Availability: Available
Subcategories: Cardiovascular Medicine
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