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Main description:
Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1970s established the non-equivalence of the maternal and paternal genomes in mammals, and a similar conclusion was drawn from studies of interploidy crosses of flowering plants that extend back to at least the 1930s. Further mouse genetic studies, involving animals carrying balanced translocations (reviewed in Chapter 3), indicated that imprinted genes were likely to be widely scattered and would form a minority within the mammalian genome. The first imprinted genes were identified in the early 1990s; over forty are now known in mammals and the list continues steadily to expand.
Contents:
Generation of Monoparental Embryos for Investigation into Genomic Imprinting
Wendy L. Dean, Gavin Kelsey, and Wolf Reik
Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting
Jeffrey R. Mann
Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome
Anne C. Ferguson-Smith, Maxine Tevendale, Pantelis Georgiades, and Valerie Grandjean
Production of YAC Transgenic Mice by Pronuclear Injection
Justin F.-X. Ainscough, Rosalind M. John, and Sheila C. Barton
A Transgenic Approach to Studying Imprinted Genes: Modified BACs and PACs
Rosalind M. John, Justin F.-X. Ainscough, and Sheila C. Barton
Methylation-Sensitive Genome Scanning
Izuho Hatada and Tsunehiro Mukai
Subtraction-Hybridization Method for the Identification of Imprinted Genes
Fumitoshi Ishino, Yoshimi Kuroiwa, Naoki Miyoshi, Shin Kobayashi, Takashi Kohda, and Tomoko Kaneko-Ishino
Identification of Imprinted Loci by Methylation: Use of Methylation- Sensitive Representational Difference Analysis (Me-RDA)
Rachel J. Smith and Gavin Kelsey
Ribonuclease Protection
Joanne L. Thorvaldsen and Marisa S. Bartolomei
Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression
Judith Singer-Sam and Chunguang Gao
Allele-Specific In Situ Hybridization (ASISH)
Rolf Ohlsson, Kristian Svensson, Hengmi Cui, Helena Malmikumpu, and Gail Adam
RNA-FISH to Analyze Allele-Specific Expression
Giovanna Braidotti
Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes
Janine LaSalle and Marc Lalande
Southern Analysis Using Methyl-Sensitive Restriction Enzymes
Tom Moore
A PCR-Based Method for Studying DNA Methylation
Mira Ariel
Bisulfite-Based Methylation Analysis of Imprinted Genes
Sabine Engemann, Osman El-Maarri, Petra Hajkova, Joachim Oswald, and Joern Walter
Direct Analysis of Chromosome Methylation
Deborah Bourc'his and Evani Viegas-Pequignot
In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs
Ilse Van den Wyngaert, Roger L. P. Adams, and Stefan U. Kass
In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation
Ghislaine Dell, Marika Charalambous, and Andrew Ward
Detection of Methyl-Sensitive DNA-Binding Proteins with Possible Involvement in the Imprinting Phenomenon
Kerstin Otte and Bjoern Rozell
Probing Chromatin Structure with Nuclease Sensitivity Assays
Richard I. Gregory, Sanjeev Khosla, and Robert Feil
Examining Histone Acetylation at Specific Genomic Regions
Ji-Fan Hu and Andrew R. Hoffman
Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis
Peter L. Jones, Paul A. Wade, and Alan P. Wolffe
Reconstitution of Chromatin In Vitro
Kiyoe Ura and Yasufumi Kaneda
Genomic Imprinting in Plants
Rinke Vinkenoog, Melissa Spielman, Sally Adams, Hugh G. Dickinson, and Rod J. Scott
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 390
Weight: 664g
Availability: Available
Subcategories: Genetics
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