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Main description:
The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection. In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures. Written in the highly successful Methods in Molecular Biology (TM) series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.
Contents:
1. Introduction to Viral Vectors
James N. Warnock, Claire Daigre, and Mohamed Al-Rubeai
2. Introduction to Gene Therapy: A Clinical Aftermath
Patrice P. Denefle
3. Host Cells and Cell Banking
Glyn N. Stacey and Otto-Wilhelm Merten
4. Overview of Current Scalable Methods for Purification of Viral Vectors
Maria Mercedes Segura, Amine Kamen, and Alain Garnier
5. Methods to Construct Recombinant Adenovirus Vectors
Miguel Chillon and Ramon Alemany
6. Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus
Edwige Dormond and Amine A. Kamen
7. Manufacturing of Retroviruses
Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana Amaral, Paula M. Alves, and Ana S. Coroadinha
8. Lentiviral Vectors
Marc Giry-Laterriere, Els Verhoeyen, and Patrick Salmon
9. Adeno-Associated Viruses
Mauro Mezzina and Otto-Wilhelm Merten
10. Manufacturing of Adeno-Associated Viruses, for Example: AAV2
Haifeng Chen
11. Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses
J. Fraser Wright and Olga Zelenaia
12. Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells
Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. Mahoenen, and Seppo Yla-Herttuala
13. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors
Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein
14. Manufacture of Measles Viruses
Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, and Mark J. Federspiel
15. In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model
Cecilia Frecha, Floriane Fusil, Francois-Loic Cosset, and Els Verhoeyen
16. In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair)
Kolja Gelse and Holm Schneider
17. Ethical Consideration
Michael Fuchs
18. Clinical Trials of GMP Products in the Gene Therapy Field
Kathleen B. Bamford
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: May, 2011
Pages: 461
Weight: 1063g
Availability: Available
Subcategories: Genetics, Microbiology
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