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Main description:
In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
Contents:
Basic Investigations in Saccharomyces cerevisiae
Brendan P. G. Curran and Virginia Bugeja
Isolation of Nucleic Acids
Michelle Hanna and Wei Xiao
Purification of Yeast Peroxisomes
Ben Distel and Astrid Kragt
Isolation of Yeast Plasma Membranes
Barry Panaretou and Peter Piper
Isolation of Yeast Mitochondria
Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott
Extraction of Yeast Lipids
Roger Schneiter and Gunther Daum
Two-Dimensional Gel Electrophoresis of Total Yeast Proteins
Helian Boucherie and Christelle Monribot-Espagne
Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes
Laura Maringele and David Lydall. Analysis of Yeast Lipids, Roger Schneiter and Gunther Daum
Yeast Fluorescence Microscopy
Jiri Hasek
Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe
Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, and Sean M. Hemmingsen
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
R. Daniel Gietz and Robin A. Woods
Mutagenesis
Leslie Barbour, Michelle Hanna, and Wei Xiao
Gene Disruption in the Budding Yeast Saccharomyces cerevisiae
Johannes H. Hegemann, Ulrich Guldener, and Gabriele J. Koehler
Inducible Degron and Its Application to Creating Conditional Mutants
R. Jurgen Dohmen
Synthetic Lethal Screen
Leslie Barbour and Wei Xiao
Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
Amy Hin Yan Tong and Charles Boone
Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
Alain T. Dandjinou, Michel Larrivee, Ralf E. Wellinger, and Raymund J. Wellinger
Chromatin Assembly in a Crude Fraction From Yeast Cells
Karen M.Robinson and Michael C. Schultz
Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast
Elena Ezhkova and William P. Tansey
Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning
Zhengjian Zhang and Joseph C. Reese
Study of Transcriptional Regulation Using a Reporter Gene Assay
Yu Fu and Wei Xiao
Assessing Telomeric Phenotypes
Catherine LeBel, Michel Larrivee, Amadou Bah, Nancy Laterreur, Nancy Levesque, and Raymund J. Wellinger
Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
Zhigang Wang
Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier
Marja Makarow, Anna-Liisa Hanninen, Taina Suntio, and Ricardo Nunes Bastos
Yeast Two-Hybrid System Screening
R. Daniel Gietz
Import of Precursor Proteins Into Isolated Yeast Mitochondria
Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 392
Weight: 595g
Availability: Available
Subcategories: Biochemistry
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