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Main description:
Circadian Rhythms: Methods and Protocols is an in-depth examination of
circadian biology, presented by leading researchers in the field. This
cutting-edge collection of protocols is adaptable for research at every
level, and represents the huge strides that chronobiologists have made
over the past two decades. These robust, reproducible procedures take
into account the myriad of variables that circadian researchers must be
cognizant of while conducting their research. By distilling the
arsenal of methodologies available, and presenting essential procedures
in precise detail, Circadian Rhythms: Methods and Protocols supports a
vast majority of circadian research efforts.
Part I of this seven-part volume provides a concise introduction to
essential components of circadian biology, including light,
photoreceptors, circadian clocks, and statistical analysis. Part II
presents protocols and techniques for monitoring and analyzing rhythmic
patterns of several model organisms. Parts III, IV, and V emphasize
the identification of clock genes and protein and RNA expression in
circadian biology. Protocols for in vitro systems comprise Part VI,
and Part VII provides an outline of methods and tips for microscopy
analysis. Circadian biologists at all research levels will realize
tremendous benefit from this extraordinary collection of methods and
protocols for the study of circadian rhythms.
Feature:
Methods for analysis of rhythmic readouts in select model organisms
Novel techniques for identifying clock genes by mutagenesis and mircroarrays
Procedures for isolation, extraction, and analysis of RNA
Procedures for extraction, fractionation, and purification of proteins
Detailed in vitro protocols for circadian research
Methods for microscopy analysis in circadian biology
Contents:
Preface
Contributors
I. Overviews.
1. Light, photoreceptors and circadian clocks
Russell G. Foster, Mark W. Hankins and Stuart N. Peirson
2. Statistical analysis of biological rhythm data
Harold B. Dowse
II. Rhythmic Readouts.
3. Rhythmic conidiation in Neurospora crassa.
Cas Kramer
4. Monitoring and analyzing Drosophila circadian locomotor activity.
Mauro A. Zordan, Clara Benna and Gabriella Mazzotta.
5. Automated video image analysis of larval zebrafish locomotor rhythms.
Gregory M. Cahill
6. Locomotor activity in rodents.
Gianluca Tosini
7. Analysis of circadian leaf movement rhythms in Arabidopsis thaliana.
Kieron D. Edwards and Andrew J. Millar
8. Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.
Shannon R. Canales, Jayna L. Ditty, Eugenia M. Clerico and Susan S. Golden
9. Analysis of rhythmic gene expression in adult Drosophila using the firefly-luciferase reporter gene.
Ralf Stanewsky
10. Monitoring circadian rhythms in Arabidopsis thaliana using luciferase reporter genes.
Anthony Hall and Paul Brown
III. Identification of Clock Genes.
11. Specialized techniques for site directed mutagenesis in cyanobacteria
Eugenia M. Clerico, Jayna L. Ditty and Susan S. Golden
12. Novel strategies for the identification of clock genes in Neurospora with insertional mutagenesis
Kruno Sveric, Moyra Mason, Till Roenneberg and Martha Merrow
13. Mutagenesis with Drosophila
Patrick Emery
14. Mutagenesis in Arabidopsis.
Jodi Maple and Simon G. Møller
15. Yeast-two-hybrid screening.
Jodi Maple and Simon G. Møller
16. Microarrays: quality control and hybridization protocol.
Ken-ichiro Uno and Hiroki R. Ueda
17. Microarrays:statistical methods for circadian rhythms.
Rikuhiro Yamada and Hiroki R. Ueda
18. Identification of clock genes using difference gel electrophoresis (DIGE).
Natasha A. Karp and Kathryn S. Lilley
IV. Gene Expression: RNA.
19. Isolation of total RNA from Neurospora mycelium.
Cas Kramer
20. RNA extraction from Drosophila heads.
Patrick Emery
21. Extraction of plant RNA.
Michael G. Salter and Helen E. Conlon
22. RNA Extraction from mammalian tissues.
Stuart N. Peirson and Jason N. Butler
23. Northern analysis of sense and antisense frequency RNA in Neurospora crassa.
Cas Kramer
24. RNase protection assay.
Patrick Emery
25. Quantitative PCR.
Stuart N. Peirson and Jason N. Butler
V. Gene Expression: Proteins.
26. Protein extraction, fractionation and purification from cyanobacteria.
Natalia B. Ivleva and Susan S. Golden
27. Protein extraction from Drosophila heads.
Patrick Emery
28. Plant protein extraction.
Helen E. Conlon and Michael G. Salter
29. Protein extraction from mammalian tissues.
Choogon Lee
30. Western blotting.
Choogon Lee
31. Co-immunoprecipitation (coIP) assay.
Choogon Lee
32. In vitro phosphorylation and kinase assays in Neurospora crassa.
Lisa Franchi, Giuseppe Macino
VI. In vitro Systems.
33. Basic protocols for Drosophila S2 cell line: maintenance and transfection
M. Fernanda Ceriani
34. Co-immunoprecipitation on Drosophila cells in culture
M. Fernanda Ceriani
35. Basic protocols for zebrafish cell lines: maintenance and transfection.
Daniela Vallone, Cristina Santoriello, Srinivas Babu Gondi and Nicholas S. Foulkes
36. Manipulation of mammalian cell lines for circadian studies.
Filippo Tamani
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: February, 2007
Pages: 578
Weight: 2290g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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