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Main description:
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Contents:
1. The Biochemical Properties of Antibodies and their Fragments
Robert M. Hnasko
2. Hybridoma Technology
Robert M. Hnasko and Larry H. Stanker
3. Affinity Purification of Antibodies
Robert M. Hnasko and Jeffery A. McGarvey
4. Bio conjugation of Antibodies to Horseradish Peroxidase (HRP)
Robert M. Hnasko
5. Indirect ELISA
Alice V. Lin
6. Direct ELISA
Alice V. Lin
7. A Double-Sandwich ELISA for Identification of Monoclonal Antibodies Suitable for Sandwich Immunoassays
Larry H. Stanker and Robert M. Hnasko
8. ELISpot and DC-ELISspot Assay to Measure Frequency of Antigen-Specific IFNg Secreting Cells
Marcelo A. Navarrete
9. The Western Blot
Thomas S. Hnasko and Robert M. Hnasko
10. Development of Flow Through Assay for Detection of Antibodies to Taenia solium Using Protein-A Colloidal Gold Conjugate as a Probe
Chennuru Sreedevi and Panduranga Rao Pavuluri
11. Multiplexed Microsphere Suspension Array-Based Immunoassays
Andrew Lin, Alexandra Salvador, and J. Mark Carter
12. Multiplex Immunoassay: A Planar Array on a Chip using the MagArray (TM) Technology
Laurie M. Clotilde, Heng Yu, and M. Luis Carbonell
13. Lateral Flow Immunoassay
Kathryn H. Ching
14. Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time
Xiaohua He and Stephanie A. Patfield
15. In-situ Proximity Ligation Assay (PLA)
Sonchita Bagchi, Robert Fredriksson, and Asa Wallen-Mackenzie
16. Tyramide Signal Amplification for Immunofluorescent Enhancement
Lauren Faget and Thomas S. Hnasko
17. Correlative microscopy for Localization of Proteins In Situ: Pre-Embedding Immuno-Electron Microscopy using FluoroNanogold, Gold Enhancement and Low-Temperature Resin
Daniela Boassa
18. Multiplex ELISA using Oligonucleotide Tethered Antibodies
Robert S. Matson
19. Gas Plasma Surface Chemistry for Biological Assays
Khoren Sahagian and Mikki Larner
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: July, 2015
Pages: 190
Weight: 6295g
Availability: Available
Subcategories: Immunology
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