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Main description:
Molecular biology has driven a powerful reductionist, or “molecule-c- tric,” approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of “naïve reductionism. ” Red- tionist methods will continue to be an essential element of all biological research efforts, but “naïve reductionism,” the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2–5). The “systems” of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or “emergent” properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e.
Contents:
An Overview of C. elegans Biology
Kevin Strange
Comparative Genomics in C. elegans, C. briggsae and Other Caenorhabditis Species
Avril Coghlan, Jason E. Stajich and Todd W. Harris
WormBase: Methods for Data Mining and Comparative Genomics
Todd W. Harris and Lincoln D. Stein
C. elegans Deletion Mutant Screening
Robert J. Barstead and Donald G. Moerman
Insertional Mutagenesis in Caenorhabditis elegans Using the Drosophila Transposon Mos1: A Method for the Rapid Identification of Mutated Genes
Jean-Louis Bessereau
SNP Mapping
M. Wayne Davis and Marc Hammarlund
Creation of Transgenic Lines Using Microparticle Bombardment Methods
Vida Praitis
Construction of Plasmids for RNAi and In Vitro Transcription of dsRNA
Lisa Timmons
Delivery Methods for RNAi in Caenorhabditis elegans
Lisa Timmons
Functional Genomic Approaches in C. elegans
Todd Lamitina
Assays for Toxicity Studies in C. elegans with Bt Crystal Proteins
Larry J. Bischof, Danielle L. Huffman and Raffi V. Aroian
Fluorescent Reporter Methods
Harald Hutter
Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans
Michael M. Francis and Andres Villu Maricq
Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis
Michael A. Miller
Preservation of C. elegans Tissue via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immuno-Cytochemistry
Robby M. Weimer
Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants
Keith Nehrke
Automated Imaging of C. elegans Behavior
Christopher J. Cronin, Zhaoyang Feng and William R. Schafer
Intracellular Ca2+ Imaging in Caehorhabditis elegans
Rex A. Kerr and William R. Schafer
In Vitro Culture of C. elegans Somatic Cells
Kevin Strange and Rebecca Morrison
Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS Flow Sorting System
Rock Pulak et al.
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: August, 2006
Pages: 292
Weight: 610g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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