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Receptor Signal Transduction Protocols
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Main description:

Gaining an understanding of the mechanisms by which cells process and respond to extracellular cues has become a major goal in many areas of bi- ogy and has attracted the attentions of almost every traditional discipline within the biological sciences. At the heart of these divergent endeavors are common methods that can aid biochemists, physiologists, and pharmacologists in ta- ling the specific questions addressed by their research. In Receptor Signal Transduction Protocols, a diverse array of meth- ologies employed to interrogate ligand--receptor and receptor-effector int- actions are described by authors who have devised and successfully applied them. The authors blend excellent descriptions and applications of fairly well established methodologies with new technologies at the cutting-edge of signal transduction research and as such I hope the present volume will complement and extend a previous excellent volume in this series edited by David Kendall and Stephen Hill (Methods Molecular Biology, vol. 41, Signal Transd- tion Protocols).


Contents:

Methods for Characterizing Receptors.

Radioligand-Binding Methods for Membrane Preparations and Intact Cells
Mary Keen

Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding
Mark E. Olah and Gary L. Stiles

Approaches to the Stable Transfection of G Protein-Coupled Receptors
Andrea Townsend-Nicholson

Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels
Hartmut Luddens and Esa R. Korpi

The Generation of Receptor-Selective Antibodies
John J. Mackrill

In Situ Hybridization
Judith C.W. Mak and Peter J. Barnes

Immunocytochemical Methods for Investigating Receptor Localization
Maria I. Fonseca and R. Dale Brown

Methods for Studying Receptor Coupling Mechanisms.

Measurement of Agonist-Stimulated [35S]GTPgS Binding to Cell Membranes
Sebastian Lazareno

Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPgS Binding
Laura J. Sim, Dana E. Selley, and Steven R. Childers

Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes
Yuji Odagaki and Kjell Fuxe

Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling
Ethan S. Burstein, Tracy A. Spalding, and Mark R. Brann

Identification and Quantitation of G Protein a-Subunits
Ian Mullaney and Graeme Milligan

Covalent Modification of G Proteins by Affinity Labeling
Martin Hohenegger, Michael Freissmuth, and Christian Nanoff

Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection
Stephen R. Ikeda

Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identity G Protein Heterotrimers Activating PhospholipaseC
Frank Kalkbrenner, Edgar Dippel, Matthias Schroff, Burghardt Wittig, and Gunter Schultz

Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons
Fe C. Abogadie, Yvonne Vallis, Noel J. Buckley, and Malcolm P. Caulfield

Methods for Investigating the Regulation of Receptor Function

Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation
Andrew B. Tobin

Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation
Alison W. Gagnon and Eamonn Kelly

Radioligand Binding Measurements of Receptor Sequestration in Intact and Permeabilized Cells
Diana M. Slowiejko and Stephen K. Fisher

Regulation of Receptor Expression: Analysis of Receptor mRNA and Gene Transcription
Philip A. Iredale, Maura E. Charlton, John D. Alvaro, and Ronald S. Duman

Index.


PRODUCT DETAILS

ISBN-13: 9781489942838
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2013
Pages: 275
Weight: 436g
Availability: Available
Subcategories: Neuroscience
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