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Main description:
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
Feature:
Contains protocols useful for both basic researchers and for those interested in applied viral gene therapy
Includes viral methods for terminally differentiated cells and for undifferentiated cells, including human stem cells
Provides example applications for many different viral vectors including HIV, herpes virus, pox virus, cytomegalovirus, adenovirus and adeno-associated viruses, baculovirus, SARS, and many others
Examines state-of-the-art imaging techniques including in vivo two-color fluorescence video micrsocopy and the combination of fluorescence imaging with PET, SPECT or MRI
Describes viral applications applicable for Medicine, Neuroscience, Immunology, Agriculture and Laboratory Safety
Back cover:
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
Contents:
1 Using Fluorescent Proteins to Study Poxvirus Morphogenesis
Brian M. Ward
2 Application of Reporter Gene Imaging for Studying Human Embryonic Stem Cells in Living Subjects
Kazim H. Narsinh, Feng Cao, and Joseph C. Wu
3 Use of a GFP-PML-expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection
Tomonori Ueno and Kiyoko Ogawa-Goto
4 Dual-Color Imaging of Tumor Angiogenesis
Robert M. Hoffman
5 Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System
Alan R. Harvey, Erich Ehlert, Joris de Wit, Eleanor S. Drummond, Margaret A. Pollett, Marc Ruitenberg, Giles W. Plant, Joost Verhaagen, and Christiaan Levelt
6 Expression of EGFP by Adenovirus-mediated Gene Transfer in the Central Nervous System
Ryohei Tomioka
7 Adenovirus-mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes
Andreas Rinne, Christoph Littwitz, Kirsten Bender, Marie-Cecile Kienitz, and Lutz Pott
8 Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry
Michael Grusch, Annemarie Losert, Andreas Lackner, Alev Deli, Irene Herbacek, and Klaus Holzmann
9 Production of Multicistronic HIV-1 Based Lentiviral Vectors
Nachimuthu Chinnasamy, James Shaffer, and Dhanalakshmi Chinnasamy
10 Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps
Claude Bagnis, Pascal Bailly, and Sylvie Chapel-Fernandes
11 Use of a Macrophage-tropic GFP-tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs
Amanda M. Brown
12 GFP-Lentiviral Vectors Targeting for NeuroAIDS
Yuanan Lu
13 Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery
Aaron M. Jasnow, Donald G. Rainnie, Kimberly A. Maguschak, Jasmeer P. Chhatwal, and Kerry J. Ressler
14 Selective Protein Expression Within the CNS Using Hybrid Lentivirus
Fredrik Gussing, Ingrid van Marion, Igor Ralets, and Cecilia Lundberg
15 Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein
Bruce W. Banfield and Gregory A. Bird
16 The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication
Travis J. Taylor and David M. Knipe
17 Modified Bovine Herpesvirus 1 for Protein Secretion
Günther M. Keil
18 Identification of Viral Peptide Fragments for Vaccine Development
Zhanglin Lin, Shuang Li, and Yong Chen
19 Use of GFP to Investigate Expression of Plant-derived Vaccines
Hong-Ye Li and Mee-Len Chye
20 Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions
Sophie Haupt, Angelika Ziegler, Graham Cowan, and Lesley Torrance
21 Sendai Virus for Cancer Immunotherapy
Yasuji Ueda, Mamoru Hasegawa, and Yoshikazu Yonemitsu
22 Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells
Qigui Yu, Ningjie Hu, and Mario Ostrowski
23 Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity
Misako Yoneda, Kentaro Fujita, Hiroki Sato, and Chieko Kai
24 Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells
Chien-Tai Tsai, Ching-Kuang Chuang, and Yu-Chen Hu
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: April, 2009
Pages: 315
Availability: Not available (reason unspecified)
Subcategories: General Issues
From the same series
CUSTOMER REVIEWS
From the reviews:
"One of the most useful modifications that have been made to viruses is the ability to encode tracers such as green fluorescent protein (GFP) … thereby allowing not only study of the virus itself, but also the behaviour of infected cells. As the title indicates, it is a methods text, giving useful practical details important to those attempting to carry out similar studies." (Christopher Ring, Microbiology Today, November, 2009)
"This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized into 24 chapters dealing with the diverse viral delivery systems for green fluorescent proteins. … The book is written primarily for practicing scientists involved in the study of green fluorescent expression in live cells and experimental animals. … provides a number of updated protocols for the practicing investigator. … a valuable companion for all researchers involved in the use of green fluorescent proteins in biological systems." (Ruben Mestril, Doody’s Review Service, September, 2009)
“This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants … . The significance of viral applications of fluorescent proteins is well covered … . it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of life sciences.” (Johannes Schmid, ChemBioChem, Vol. 10, 2009)
“Cover the use of Green Fluorescent Protein (GFP) technology to study aspects of the replication of viruses and the majority of the chapters describe the use of viruses and GFP as research tools in a much wider context. … I enjoyed the book for the breadth of topics and snapshots of research … . a more likely home for this book is a university or institute library where its eclectic contents will have a sufficiently wide audience.” (Paul Monaghan, Infocus Magazine, Issue 19, September, 2010)