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Main description:
There have been significant advances in research involving the isolation and culture of epithelial cells in the past decade, and many new techniques have been developed. Monolayer cultures can be used to evaluate the nature and behavior of cells, while the use of epithelial cells in model systems has allowed a deeper understanding of cellular and molecular mechanisms and interactions. The aim of this book is to provide a comprehensive, step-by-step guide to many techniques for epithelial cell culture, combining in one volume the more commonly used protocols along with many that are more speci- ized. Epithelial Cell Culture Protocols should help those who are new to this field and want to learn the basic culture techniques, as well as those needing to use more wide ranging and specific protocols. It should be a useful resource on its own, and also complement the other volumes that have been written about cell culture in the Methods in Molecular Biology series. Epithelial Cell Culture Protocols covers a wide variety of protocols, mostly aimed at the researcher, but also a few aimed at clinicians. The est- lishment and maintenance of primary cultures derived from many different tissues and different species is covered. Particular emphasis has been placed on protocols needed to further analyze and assess epithelial cells, for example, by looking at apoptosis and integrins and by measuring membrane capa- tance and confluence. Using different co-culture techniques, it is possible also to develop models to investigate many different systems in vitro.
Contents:
Human Lens Epithelial Cell Culture
Nobuhiro Ibaraki
Human Airway Epithelial Cell Culture
Mutsuo Yamaya, Masayoshi Hosoda, Tomoko Suzuki, Norihiro Yamada, and Hidetada Sasaki
Rat Gastric Mucosal Epithelial Cell Culture
Yoshitaka Konda and Tsutomu Chiba
Thymic Epithelial Cell Culture
Carsten Roepke
Bile Duct Epithelial Cell Culture
Alphonse E. Sirica
Liver Epithelial Cell Culture
Rolf Gebhardt
Pulmonary Epithelial Cell Culture
Ben Forbes
Prostate Epithelial Cell Isolation and Culture
David L. Hudson
A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier
Albert Guidry and Celia O'Brien
The Blood-CSF Barrier in Culture: Development of a Primary Culture and Transepithelial Transport Model for Choroidal Epithelial Cells
Wei Zheng and Qiuqu Zhao
An In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary Cultures
Philip H. Karp, Thomas O. Moninger, S. Pary Weber, Tamara S. Nesselhauf, Janice L. Launspach, Joseph Zabner, and Michael J. Welsh
Primary Mouse Keratinocyte Culture
Kairbaan Hodivala-Dilke
Analyzing Apoptosis in Cultured Epithelial Cells
Andrew P. Gilmore and Charles H. Streuli
Keratins as Markers of Epithelial Cells
David L. Hudson
Epithelial Cell Integrins
Joachim Rychly
Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium
Sebastian Zellmer and Dieter Reissig
Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces
Norio Kumagai
Transplantation of Cultured Epithelial Cells
Minoru Ueda, Yukio Sumi, Yoshitaka Hibino, and Ken-Ichiro Hata
An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Modelto Study the Coordination of Cellular Migration and Proliferation
Tohru Masui
Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract
Jie Shen and Vincent H. L. Lee
Applications of Epithelial Cell Culture in Studies of Drug Transport
Staffan Tavelin, Johan Grasjoe, Jan Taipalensuu, Goeran Ocklind, and Per Artursson
X-Ray Microanalysis of Epithelial Cells in Culture
Godfried M. Roomans
ENU Mutagenesis of Rat Mammary Epithelial Cells
George Stoica
ENU-Induced Ovarian Cancer
George Stoica
Measurement of Membrane Capacitance in Epithelial Monolayers
Carol A. Bertrand and Ulrich Hopfer
Assessing Epithelial Cell Confluence by Spectroscopy
Simon A. Lewis
Co-Cultivation of Liver Epithelial Cells with Hepatocytes
Rolf Gebhardt
Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium
Wee Soo Shin, Chieko Hemmi, and Teruhiko Toyo-oka
Establishing Epithelial-Immune Cell Co-Cultures: Effects on Epithelial Ion Transport and Permeability
Derek M. McKay and Mary H. Perdue
Explant Cultures of Embryonic Epithelium: Analysis of Mesenchymal Signals
Carin Sahlberg, Tuija Mustonen, and Irma Thesleff
Bacterial Interactions with Host Epithelium In Vitro
Nicola Jones, Mary H. Perdue, Philip M. Sherman, and Derek M. McKay
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 420
Weight: 710g
Availability: Available
Subcategories: Respiratory Medicine
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