(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.
Contents:
Part I: Protein Conjugates
1. Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities
Edward A. Lemke
2. Enzymatically-Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase
Ahmed Besheer, Thomas C. Hertel, Joerg Kressler, Karsten Mader, and Markus Pietzsch
3. Synthesis of Drug/Dye-Incorporated Polymer-Protein Hybrids
Sukanta Dolai, Wei Shi, Bikash Mondal, and Krishnaswami Raja
4. Dye/DNA Conjugates as Multiple Labels for Antibodies in Sensitive Fluorescence Immunoassays
Qin Zhang, Shengchao Zhu, and Liang-Hong Guo
5. Chemoselective Modification of Viral Proteins Bearing Metabolically Introduced 'Clickable' Amino Acids and Sugars
Partha S. Banerjee and Isaac S. Carrico
6. Preparation of Peptide and Other Biomolecular Conjugates through Chemoselective Ligations
Mathieu Galibert, Olivier Renaudet, Didier Boturyn, and Pascal Dumy
7. New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed Covalent Protein Labeling
Noriho Kamiya and Hiroki Abe
8. Covalent Conjugation of Poly(Ethylene Glycol) to Proteins and Peptides: Strategies And Methods
Anna Mero, Chiara Clementi, Francesco M. Veronese, and Gianfranco Pasut
9. Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag to Proteins Using Protein Trans-Splicing
Tulika Dhar, Thomas Kurpiers, and Henning D. Mootz
Part II: Nucleic Acid Conjugates
10. Polyethylenimine Bioconjugates for Imaging and DNA Delivery In Vivo
Andrea Masotti and Francesco Pampaloni
11. Synthesis of a Glycomimetic Oligonucleotide Conjugate By 1,3-Dipolar Cycloaddition
Gwladys Pourceau, Albert Meyer, Jean-Jacques Vasseur, and Francois Morvan
12. Site-Specific DNA Labeling by Staudinger Ligation
Samuel H. Weisbrod, Anna Baccaro, and Andreas Marx
13. Improved Cellular Uptake of Antisense Peptide Nucleic Acids (PNAs) by Conjugation to a Cell-Penetrating Peptide and a Lipid Domain
Takehiko Shiraishi and Peter E. Nielsen
14. Synthesis of Oligonucleotide-Peptide Conjugates for Biomedical and Technological Applications
Anna Avino, Santiago Grijalvo, Sonia Perez-Rentero, Alejandra Garibotti, Montserrat Terrazas, and Ramon Eritja
15. Amphiphilic DNA Block Copolymers: Nucleic Acid-Polymer Hybrid Materials for Diagnostics and Biomedicine
Jan Zimmermann, Minseok Kwak, Andrew J. Musser, and Andreas Herrmann
Part III: Glycosyl and Lipid Conjugates
16. Chemically Selective Liposome Surface Glyco-Functionalization
Hailong Zhang, Yong Ma, and Xue-Long Sun
17. Bioconjugation Using Mutant Glycosyltransferases for the Site-Specific Labeling of Biomolecules with Sugars Carrying Chemical Handles
Boopathy Ramakrishnan, Elizabeth Boeggeman, Marta Pasek, and Pradman K. Qasba
18. Lipid-Core-Peptide System for Self-Adjuvanting Synthetic Vaccine Delivery
Mariusz Skwarczynski and Istvan Toth
19. Coupling Carbohydrates to Proteins for Glycoconjugate Vaccine Development Using a Pentenoyl Group as a Convenient Linker
Qianli Wang and Zhongwu Guo
20. Conjugation of LPS-Derived Oligosaccharides to Proteins Using Oxime Chemistry
Joanna Kubler-Kielb
21. Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
Junpeng Xiao and Thomas J. Tolbert
22. On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-linked Oligosaccharide
Rui Chen and Thomas J. Tolbert
23. Design and Synthesis of Novel Functional Lipid-Based Bioconjugates for Drug Delivery and Other Applications
Rupa R. Sawant and Vladimir P. Torchilin
Part IV: Biofunctionalization of Surfaces and Thin Films
24. Chemical Functionalization and Bioconjugation Strategies for Atomic Force Microscope (AFM) Cantilevers
Magnus Bergkvist and Nathaniel C. Cady
25. Chemoselective Protein and Peptide Immobilization on Biosensor Surfaces
Edith H.M. Lempens, Brett A. Helms, and Maarten Merkx
26. Fabrication of Dynamic Self-Assembled Monolayers for Cell Migration and Adhesion Studies
Nathan P. Westcott and Muhammad N. Yousaf
27. DNA Detection Using Functionalized Conducting Polymers
Jadranka Travas-Sejdic, Hui Peng, Hsiao-hua Yu, and Shyh-Chyang Luo
28. Preparation and Dynamic Patterning Of Supported Lipid Membranes Mimicking Cell Membranes
Stefan Kaufmann, Karthik Kumar, and Erik Reimhult
29. Enzyme Immobilization on Reactive Polymer Films
Ana L. Cordeiro, Tilo Pompe, Mariana Tasso, Katrin Salchert, and Carsten Werner
30. Characterization of Protein-Membrane Binding Interactions Via a Microplate Assay Employing Whole Liposome Immobilization
Matthew D. Smith and Michael D. Best
31. A Bioconjugated Phospholipid Polymer Biointerface with Nanometer-Scaled Structure for Highly Sensitive Immunoassays
Kazuki Nishizawa, Madoka Takai, and Kazuhiko Ishihara
Part V: Biofunctionalization of Nanostructures
32. Purification, Functionalization, and Bioconjugation of Carbon Nanotubes
John H.T. Luong, Keith B. Male, Khaled A. Mahmoud, and Fwu-Shan Sheu
33. Functional Integration of Membrane Proteins with Nanotube and Nanowire Transistor Devices
Aleksandr Noy, Alexander B. Artyukhin, Shih-Chieh Huang, Julio A. Martinez, and Nipun Misra
34. Single-Step Conjugation of Antibodies to Quantum Dots for Labeling Cell Surface Receptors in Mammalian Cells
Gopal Iyer, Jianmin Xu, and Shimon Weiss
35. A Practical Strategy for Constructing Nanodrugs Using Carbon Nanotubes as Carriers
Wei Wu and Xiqun Jiang
36. Design and Synthesis of Biofunctionalized Metallic/Magnetic Nanomaterials
Eun-Kyung Lim, Seungjoo Haam, Kwangyeol Lee, and Yong-Min Huh
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: June, 2011
Pages: 624
Weight: 1368g
Availability: Available
Subcategories: Biochemistry, Biomedical Engineering
From the same series
