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Advanced Fluorescence Microscopy
Methods and Protocols
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Main description:

This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.


Feature:

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


Back cover:

This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.


Contents:

1. The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications

Natalie S. Poulter, William T.E. Pitkeathly, Philip J. Smith, and Joshua Z. Rappoport

 

2. Two-Photon Excitation Microscopy And Its Applications In Neuroscience

Ricardo Mostany, Amaya Miquelajauregui, Matthew Shtrahman, and Carlos Portera-Cailliau

 

3. Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy

Francesco Pampaloni, Roli Richa, Nariman Ansari, and Ernst H.K. Stelzer

 

4. Fluorescence Microscopy Based RNA Interference Screening

Manuel Gunkel, Nina Beil, Jürgen Beneke, Jürgen Reymann, and Holger Erfle

 

5. Fluorescence Resonance Energy Transfer Microscopy (FRET)

Kasia M. Kedziora and Kees Jalink

 

6. Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy

Yuansheng Sun, Vinod Jyothikumar, and Ammasi Periasamy

 

7. Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation

Bart Geverts, Martin E. van Royen, and Adriaan B. Houtsmuller

 

8. Fluorescence Correlation Spectroscopy

Mark A. Hink

 

9. Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules

Suvrajit Saha, Riya Raghupathy, and Satyajit Mayor

 

10. Practical Structured-Illumination Microscopy E. Hesper Rego and Lin Shao

 

11. 4Pi Microscopy of the Nuclear Pore Complex

Martin Kahms, Jana Hüve, and Reiner Peters

 

12. Application Of STED Microscopy To Cell Biology Questions

Natalia H. Revelo and Silvio O. Rizzoli

 

13. Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes

Kelsey Temprine, Andrew G. York, and Hari Shroff

 

14. Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

Ulrike Endesfelder and Mike Heilemann

 

15. Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells

Taofei Yin and Yi I. Wu

 


PRODUCT DETAILS

ISBN-13: 9781493920792
Publisher: Springer (Springer New York)
Publication date: November, 2014
Pages: 275
Weight: 814g
Availability: Not available (reason unspecified)
Subcategories: Radiology
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