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MORE ABOUT THIS BOOK
Main description:
T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.
Contents:
Part I. Isolation of T Cells from the Tissues
1. Isolation of T Cells from the Skin
Beate Lorenz and Esther von Stebut
2. Isolation of Leukocytes from Mouse Central Nervous System
Iva Lelios and Melanie Greter
3. Isolation of T Cells from the Gut
Sonja Reissig, Christopher Hackenbruch, and Nadine Hoevelmeyer
4. T Cell Isolation from Mouse Kidneys
Isis Ludwig-Portugall and Christian Kurts
Part II. Analysis of T Cell Function and Phenotypes
5. Intracellular Staining for Cytokines and Transcription Factors
Florian Mair and Vinko Tosevski
6. Surface Capture Staining
Vinko Tosevski and Florian Mair
7. Mass Cytometry Analysis of Human T Cell Phenotype and Function
Evan W. Newell and Lai Li Yun
8. Detection of NF- B Pathway Activation in T Helper Cells
Oliver Gorka, Stefan Wanninger, and Jurgen Ruland
9. Assessing the Suppressive Activity of Foxp3+ Regulatory T Cells
Christian Thomas Mayer and Tim Sparwasser
10. In vitro Generation of Microbe Specific Human Th17 Cells
Julia M. Braun and Christina E. Zielinski
11. In vitro Polarization of T-Helper Cells
Julie Rumble and Benjamin Segal
Part III. In vivo Models of T Cell Function
12. A Method for the Generation of TCR Retrogenic Mice
Elisa Kieback, Ellen Hilgenberg, and Simon Fillatreau
13. Mouse Models of Allergic Airway Disease
Helen Meyer-Martin, Sebastian Reuter, and Christian Taube
14. Induction of Colitis in Mice (T-Cell Transfer Model)
Benno Weigmann
15. Manipulation of T Cell Function Conditional Gene Targeting in T Cells
Anna Sledzinska, Lynsey Fairbairn, and Thorsten Buch
16. Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes
C.T. Wohn, S. Pantelyushin, J.L. Ober-Bloebaum, and B.E. Clausen
17. Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells
Rhoanne C. McPherson, Helen E. Cambrook, Richard A. O'Connor, and Stephen M. Anderton
18. Experimental Mouse Models of T Cell-Dependent Inflammatory Bowel Disease
George X. Song-Zhao and Kevin J. Maloy
19. Analysis of Chromosomal Aberrations in Murine HCC
Kristian Unger and Mathias Heikenwalder
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2014
Pages: 220
Weight: 6624g
Availability: Available
Subcategories: Anatomy, Immunology, Pathology
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