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MORE ABOUT THIS BOOK
Main description:
This volume focuses on malaria vaccinology, and introduces researchers to a complete overview of the protocols and tools used by molecular and cellular malariologists. The chapters within this book talk about interventions concerning all aspects of life cycle progression--measuring antibody responses to blood stage parasite survival, the T Cell responses engendered by attenuated sporozoite vaccinations, and the unique effect on transmission of antibodies that target the mosquito stage of the life cycle. Additionally, methods concerning the ability to generate targeted gene deletions and replacements in the genome of Plasmodium parasites convey how Plasmodium parasite phenotypes can be created to precise specifications. More recently, the potential power of humanized mouse models of disease progression have been demonstrated and are also discussed in the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Malaria Vaccines: Methods and Protocols is a useful and unique resource for any aspiring malaria vaccinologists because of the volume's exquisitely detailed methodologies that are not typically found in published literature.
Contents:
Part I Pre-Erythrocytic Stages
1. Isolation of Non-Parenchymal Cells from the Mouse Liver
Isaac Mohar, Katherine J. Brempelis, Sara A. Murray, Mohammad R. Ebrahimkhani, and I. Nicholas Crispe
2. Measurement of the T Cell Response to Pre-Erythrocytic Vaccination in Mice
Jenna J. Guthmiller, Ryan A. Zander, and Noah S. Butler
3. Characterization of Liver CD8 T cell Subsets that are Associated with Protection against Pre-Erythrocytic Plasmodium Parasites
Stasya Zarling and Urszula Krzych
4. Flow Cytometry-Based Assessment of Antibody Function against Malaria Pre-Erythrocytic Infection
Alyse N. Douglass, Peter G. Metzger, Stefan H.I. Kappe, and Alexis Kaushansky
5. Assessment of Parasite Liver Stage Burden in Human-Liver Chimeric Mice
Lander Foquet, Philip Meuleman, Rob Hermsen, Robert Sauerwein, and Geert Leroux-Roels
6. Measurement of Antibody-Mediated Reduction of Plasmodium Yoelii Liver Burden by Bioluminescent Imaging
Brandon K. Sack, Jessica L. Miller, Ashley M. Vaughan and Stefan H. I. Kappe
7. Detection of Plasmodium Berghei and Plasmodium Yoelii Liver-Stage Parasite Burden by Quantitative Real Time PCR
Alexander Pichugin and Urszula Krzych
Part II Mosquito Stages
8. Membrane Feeding Assay to Determine the Infectiousness of Plasmodium Vivax Gametocytes
Jetsumon Sattabongkot Prachumsri, Chalermpon Kumpitak, and Kirakorn Kiattibutr
9. The Standard
Membrane Feeding Assay: Advances Using
Bioluminescence
Will J. R. Stone and Teun Bousema
Part III Erythrocytic Stages
10. Agglutination Assays of the Plasmodium Falciparum Infected Erythrocyte
Joshua Tan and Peter C. Bull
11. Antibody-Dependent Cell-Mediated Inhibition (ADCI) of Plasmodium Falciparum: One and Two-Step ADCI Assays
Hasnaa Bouharoun-Tayoun and Pierre Druilhe
12. A Robust Phagocytosis Assay to Evaluate the Opsonic Activity of Antibodies against Plasmodium Falciparum-Infected Erythrocytes
Andrew Teo, Wina Hasang, Philippe Boeuf, and Stephen Rogerson
13. Miniaturized Growth Inhibition Assay to Assess the Anti-Blood Stage Activity of Antibodies
Elizabeth H. Duncan and Elke S. Bergmann-Leitner
14. Measuring Plasmodium Falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry
Amy Kristine Bei and Manoj T. Duraisingh
15. The In Vitro Invasion Inhibition Assay (IIA) for Plasmodium Vivax
Wanlapa Roobsoong
16. The Ex Vivo IFN- Enzyme-Linked Immunospot (ELISpot) Assay
Martha Sedegah
17. Evaluating IgG Antibody to Variant Surface Antigens Expressed on Plasmodium Falciparum Infected Erythrocytes Using Flow Cytometry
Andrew Teo, Wina Hasang and Stephen Rogerson
18. Inhibition of Infected Red Blood Cell Binding to the Vascular Endothelium
Marion Avril
19. Evaluation of Pregnancy Ma
laria V
accine
Candidates: The Binding Inhibition Assay
Tracy Saveria, Patrick E. Duffy, and Michal Fried
20. High-Throughput Testing of Antibody Dependent Binding Inhibition of Placental Malaria Parasites
Morten A. Nielsen and Ali Salanti
Part IV Parasite Manipulation
21. Generation of Transgenic Rodent Malaria Parasites Expressing Human-Malaria Parasite Proteins
Ahmed M. Salman, Catherin Marin Mogollon, Jing-wen Lin, Fiona J.A. van Pul, Chris J. Janse, and Shahid M. Khan
Part V Vaccination
22. Vaccination Using Gene-Gun Technology
Elke Bergmann-Leitner and Wolfgang W. Leitner
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2015
Pages: 238
Weight: 8185g
Availability: Available
Subcategories: Immunology, Pharmacology
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