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MORE ABOUT THIS BOOK
Main description:
The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book's thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.
Contents:
Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
Malgorzata Z. Zdzienicka
Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes
Mathilde Frechet, Valerie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, and Thierry Magnaldo
Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities
William W. Au and Salama A. Salama
Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation
Shruti Nagar, James J. Corcoran, and William F. Morgan
Inhibition of DNA Synthesis by Ionizing Radiation: A Marker for an S-Phase Checkpoint
Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka
Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
George Iliakis, Ya Wang, and Hong Yan Wang
Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage
Xuan Huang and Zbigniew Darzynkiewicz
Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication)
Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi
In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
George Iliakis and Nge Cheong
Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
Michael J. Villalobos, Christopher J. Betti, and Andrew T. M Vaughan
Plasmid-Based Assays for DNA End-Joining In Vitro
George Iliakis, Bustanur Rosidi, Minli Wang, and Huichen Wang
Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants
Rodney S. Nairn and Gerald M. Adair
Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr
Quantitative PCR-Based Measurement of Nuclear and MitochondrialDNA Damage and Repair in Mammalian Cells
Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, and Bennett Van Houten
Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR
Gerd P. Pfeifer
Immunochemical Detection of UV-Induced DNA Damage and Repair
Marcus S. Cooke and Alistair Robson
A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts
Shirley McCready
Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
David L. Mitchell
DNA Damage Quantitation by Alkaline Gel Electrophoresis
Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland
The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Gunter Speit and Andreas Hartmann
Fast Micromethod DNA Single-Strand-Break Assay
Heinz C. Schroeder, Renato Batel, Heiko Schwertner, Oleksandra Boreiko, and Werner E. G. Muller
32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC
Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki
Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA
Vaughn Smider, Byung Joon Hwang, and Gilbert Chu
Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision
Huixian Wang and John B. Hays
Characterization of Enzymes that Initiate Base Excision Repair at Abasic Sites
Walter A. Deutsch and Vijay Hegde
Base Excision Repair in Mammalian Cells
Yoshihiro Matsumoto
In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti
Biochemical Assays for the Characterization of DNA Helicases
Robert M. Brosh, Jr. and Sudha Sharma
Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 498
Weight: 754g
Availability: Available
Subcategories: Biochemistry
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