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Main description:
This volume of the Methods in Molecular Biology series is entirely devoted to the study of steroid receptor biology. Steroid hormone receptors represent a powerful system for the study of both the most fundamental molecular mec- nisms of gene regulation and control and the gross physiological responses of organisms to steroid hormones. Research in this field has brought forth advances in the treatment of cancer, endocrine disorders, and reproductive biology, and allowed elucidation of the fundamental biological mechanisms of gene expr- sion. In Steroid Receptor Methods: Protocols and Assays, the reader will find a collection of methods and protocols submitted by many fine steroid receptor researchers from throughout the world. These authors have been instructed to create a highly informative cross-section of the latest research techniques ava- able. The resulting work is timely, useful, and approachable for both the ex- rienced researcher and the novice to the field. Because the steroid receptor family is represented by a wonderfully diverse, yet strongly interrelated set of steroid receptor proteins, Steroid Receptor Methods contains protocols for the prod- tion and purification of a variety of receptor forms, including the progesterone, glucocorticoid, and androgen receptors. These procedures provide the raw ma- rial needed to conduct sophisticated biochemical analysis of receptor properties. Other techniques presented allow the reader to perform biochemical experiments on DNA binding characteristics, hormone binding assays, and protocols using combinatorial chemistry for drug discovery.
Contents:
Part I. Bioinformatics
Bioinformatics of Nuclear Receptors
Mark Danielsen
Phylogenetic Inference and Parsimony Analysis
Llewellyn D. Densmore III
Part II. Purification Protocols
Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems
Vida Senkus Melvin and Dean P. Edwards
High-Yield Purification of Functionally Active Glucocorticoid Receptor
Terace M. Fletcher, Barbour S. Warren, Christopher T. Baumann, and Gordon L. Hager
Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor
Mingmin Liao and Elizabeth M. Wilson
Large Scale Production of Nuclear Receptor Ligand-Binding Domains
Li-Zhi Mi and Fraydoon Rastinejad
Physical Structure of Nuclear Receptor-DNA Complexes
Scott A. Chasse and Fraydoon Rastinejad
Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display
Sushma Kumar, Maarit Angervo, Milan K. Bagchi, and Indrani C. Bagchi
Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes
Bruce Blumberg
Part III. Steroid Hormone-Binding Assays
Use of [99mTc] Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors
Frank Wust
Steroid Hormone Metabolites and Hormone Binding Assays
Rosemary Bland and Martin Hewison
Vitamin D3 Analog Screening
Sami Vaisanen, Sanna Ryhanen, and Pekka H. Maenpaa
Part IV. Protein Interaction Assays
Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors
Steven K. Nordeen, Paul R. Housley, Yihong Wan, and Richard N. Day
Knockout Mice and Steroid Receptor Research
Per Flodby, Stephan Teglund, andJan-Ake Gustafsson
Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors
Bertrand Le Douarin, David M. Heery, Claudine Gaudon, Elmar vom Baur, and Regine Losson
Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex
Rabindra N. Bhattacharjee, Caroline Underhill, and Joseph Torchia
Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System
Simon J. Evans and Frank L. Moore
Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay
James R. Lambert and Steven K. Nordeen
Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription In Vivo
Christy J. Fryer and Trevor K. Archer
Cotransfection Assays and Steroid Receptor Biology
Shimin Zhang and Mark Danielsen
Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System
Hironobu Sasano, Sachiko Matsuzaki, and Takashi Suzuki
Part V. Cancer Research and Drug Discovery
Solid Tumor Cancer Markers and Applications to Steroid Hormone Research
Marcia V. Fournier, Katherine J. Martin, and Arthur B. Pardee
Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay
Kathleen F. Arcaro and John F. Gierthy
Combinatorial Chemistry in Steroid Receptor Drug Discovery
John A. Flygare, Daniel P. Sutherlin, and S. David Brown
Identification of Nuclear Receptor Interacting Proteins Using Yeast Two-Hybrid Technology: Applications to Drug Discovery
Sunil Nagpal, Corine R. Ghosn, and Roshantha A. S. Chandraratna
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 396
Weight: 581g
Availability: Available
Subcategories: Biochemistry
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