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MORE ABOUT THIS BOOK
Main description:
A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contents:
Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements
Michael Aschner, Vanessa A. Fitsanakis, Ana Paula Marreilha dos Santos, Luisa Olivi, and Joseph P. Bressler
Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences
Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick
The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
Triantafyllos Chavakis and Valeria Orlova
Cell-Cell Interactions on Solid Matrices
Nancy A. Louis, Dionne Daniels, and Sean P. Colgan
Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein a-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function
Ernesto Sabath and Bradley M. Denker
Nucleotide Metabolism and Cell-Cell Interactions
Holger K. Eltzschig, Thomas Weissmuller, Alice Mager, and Tobias Eckle
Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization
Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans
Collagen Gel Contraction Assay
Peter Ngo, Punitha Ramalingam, Jonathan A. Phillips, and Glenn T. Furuta
Methods to Assess Tissue Permeability
Juan C. Ibla and Joseph Khoury
Bacterial-Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy
Kimberly K. Jefferson and Nuno Cerca
A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum
Heidi E. De Luca and Wayne I. Lencer
Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
Yaw-Chyn Lim and Francis W. Luscinskas
Expression Cloningof Signaling Proteins Regulated by Cell Adhesion
Michelle L. Matter and Joe W. Ramos
Assays for the Calcium Sensitivity of Desmosomes
Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya
Tight Junctions and Cell-Cell Interactions
Markus Utech, Matthias Bruwer, and Asma Nusrat
Simultaneous Immunofluorescence Detection of Cadherin and b-Catenin in Mouse Tissue Sections
Nobuko Obara
In Vitro Neutrophil Transepithelial Migration
Winston Y. Lee, Alex C. Chin, Susan Voss, and Charles A. Parkos
The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions
Robert Sackstein and Robert Fuhlbrigge
Cell-Cell Interaction in the Transcellular Biosynthesis of Novel w-3-Derived Lipid Mediators
Nan Chiang and Charles N. Serhan
Isolation and Analysis of Lipid Rafts in Cell-Cell Interactions
Aimee Landry and Ramnik Xavier
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2006
Pages: 304
Weight: 686g
Availability: Available
Subcategories: General Issues, Microbiology
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