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Main description:
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
Feature:
Brings together all the methodology needed to perform human hepatocyte transplantation for the first time in one volume
Key researchers cover all aspects of hepatocyte transplantation from bench to bedside
Provides a practical basis for evaluating stem cell-derived hepatocytes for the treatment of liver disease in the future
Back cover:
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
Contents:
1. Human Hepatocyte Transplantation Overview
Juliana Puppi and Anil Dhawan
2. Isolation of Human Hepatocytes
Ragai R. Mitry
3. An Optimised Method for Cryopreservation of Human Hepatocytes
Claire Terry and Robin D. Hughes
4. Liver Cell Culture Techniques
José V. Castell and Maria José Gómez-Lechón
5. In vitro Assays for Induction of Drug Metabolism
Brian G. Lake, Roger J. Price, Amanda M. Giddings and David G. Walters
6. Hepatocyte Apoptosis
Mustapha Najimi, Françoise Smets and Etienne Sokal
7. Small Animal Models of Hepatocyte Transplantation
Jurgen Seppen, Ebtisam El Filali and Ronald Oude Elferink
8. Hepatocyte Transplantation Techniques: Large Animal Models
Anne Weber, Marie-Thérèse Groyer-Picard and Ibrahim Dagher
9. Cell Transplant Techniques: Engraftment Detection of Cells
Robert A. Fisher and Valeria R. Mas
10. Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation
Yao-Ming Wu and Sanjeev Gupta
11. Ex vivo Gene Transfer into Hepatocytes
Xia Wang, Prashant Mani, Debi P. Sarkar, Namita Roy-Chowdhury and Jayanta Roy-Chowdhury
12. Sources of Adult Hepatic Stem Cells: Haematopoietic
Rosemary Jeffery, Richard Poulsom and Malcolm R. Alison
13. Production of Hepatocyte-like Cells from Human Amnion
Toshio Miki, Fabio Marongiu, Ewa C.S. Ellis, Ken Dorko, Keitaro Mitamura, Aarati Ranade, Roberto Gramignoli and Stephen C. Strom
14. Generation of Hepatocytes from Human Embryonic Stem Cells
Niloufar Safinia and Stephen L. Minger
15. Isolation, In vitro Cultivation and Characterisation of Foetal Liver Cells
Yue Wu, Chetan C. Shatapathy and Stephen L. Minger
16. Human Intrahepatic Biliary Epithelial Cell Lineages: Studies in vitro
Ruth Joplin and Stivelia Kachilele
17. Liver Cell Labelling with MRI Contrast Agents
Michel Modo, Thomas J. Meade and Ragai R. Mitry
18. Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory
Sharon C. Lehec
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: January, 2009
Pages: 231
Weight: 764g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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