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Main description:
Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology (TM) series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls.
Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.
Contents:
1. The Expanding World of Small Molecule Microarrays
Mahesh Uttamchandani and Shao Q. Yao
2. Construction of Photo-Cross-Linked Microarrays of Small Molecules
Naoki Kanoh
3. Small Molecule Microarray: Functional-Group Specific Immobilization of Small Molecules
Hyang Yeon Lee and Seung Bum Park
4. Detecting Protein-Small Molecule Interactions Using Fluorous Small-Molecule Microarrays
Arturo J. Vegas and Angela N. Koehler
5. A Method for Small Molecule Microarray (SMM)-Based Screening for the Rapid Discovery of Affinity-Based Probes (AfBPs)
Haibin Shi, Mahesh Uttamchandani, and Shao Q. Yao
6. Nanodroplet Chemical Microarrays and Label-Free Assays
Dhaval Gosalia and Scott L. Diamond
7. Nanodroplet Microarrays for High-Throughput Enzyme Screening
Kang L.D. Aw, Shao Q. Yao, and Mahesh Uttamchandani
8. The Application of the Chemical Array for Biological Study
Isao Miyazaki, Siro Simizu, and Hiroyuki Osada
9. Peptide Arrays with a Chip
Alexander Nesterov, Edgar Doersam, Yun-Chien Cheng, Christopher Schirwitz, Frieder Markle, Felix Loeffler, Kai Koenig, Volker Stadler, Ralf Bischoff, and Frank Breitling
10. In situ Chemical Modification of Peptide Microarrays: Characterization by Desorption/Ionization on Silicon Nanowires
Gaelle Piret, Herve Drobecq, Rabah Boukherroub, and Oleg Melnyk
11. In situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated Antigens
Remi Desmet, Eric Diesis, Herve Drobecq, Carine Rouanet, Karim Chemlal, Anne-Sophie Debrie, Jean-Michel Hougardy, Francoise Mascart, Camille Locht, and Oleg Melnyk
12. Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection
Marina Cretich, Francesco Damin, Renato Longhi, Cecilia Gotti, Clelia Galati, Lucio Renna, and Marcella Chiari
13. Antibody Signatures Defined by High-Content Peptide Microarray Analysis
Antonia Masch, Johannes Zerweck, Ulf Reimer, Holger Wenschuh, and Mike Schutkowski
14. HighDensity Peptide Microarrays for Proteome-Wide Fingerprinting of Kinase Activities in Cell Lysates
Alexandra Thiele, Matthias Weiwad, Johannes Zerweck, Gunter Fischer, and Mike Schutkowski
15. A Peptide Microarray for Detecting Protein Kinase Activity in Cell Lysates
Xiaoming Han and Yoshiki Katayama
16. Chemical Microarrays Constructed by Selective Attachment of Hydrazide-Conjugated Substances to Epoxide Surfaces and Their Applications
Sungjin Park, Myung-Ryul Lee, and Injae Shin
17. A Microarray-Based Method to Perform Nucleic Acid Selections
Olga Aminova and Matthew D. Disney
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2010
Pages: 226
Weight: 711g
Availability: Available
Subcategories: Biochemistry, Genetics
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