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Main description:
Proteins have evolved through selective pressure to accomplish specific functions. The functional properties of proteins depend upon their thr- dimensional structures, which result from particular amino acid sequences folding into tightly packed domains. Thus, to understand and modulate protein function rationally, one definitely needs methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. Protein design aims precisely at providing the tools to achieve this goal. The predictive power of rational protein design methods has dramatically increased over the past five years. A broad range of studies now illustrate how the sequence of proteins and peptides can be tuned to engineer biological tools with intended properties (1–3). The extensive characterization of peptides and protein mutants has enormously benefited the understanding of protein sequence-to-structure relationships. Synergies between computational and experimental approaches have also added momentum to the advancing limits of design methods. The potential applications in fundamental biochemistry and in biotechnology justify the considerable excitement that this progress has generated within the research community. The field is probably mature enough so that expert knowledge can assist researchers of diverse disciplines to rationally create or modify their favorite protein. Thus, the aim of Protein Design: Methods and Protocols is to account for the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with maximum confidence in a successful return. The basic concepts underlying rational design of proteins are intimately related to their three-dimensional structures.
Back cover:
Recent major advances in our understanding of modulating protein functions has led to the development of new methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success.
The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins. Other topics of discussion include: design of structural elementary motifs, entire proteins, and interfaces of protein complexes, and of amyloidogenic polypeptides and amyloid inhibitors.
Authoritative and cutting-edge, Protein Design: Methods and Applications will be of major interest to protein scientists, biochemists, and all experimentalists selecting the strategy most adaptable for their design problem.
Contents:
I. Design of Structural Elementary Motifs
Structure and Stability of the a-Helix: Lessons for Design
Neil Errington, Teuku Iqbalsyah, and Andrew J. Doig
De novo Design of Monomeric b-Hairpin and b-Sheet Peptides
David Pantoja-Uceda, Clara M. Santiveri, and M. Angeles Jiménez
De novo Proteins From Binary-Patterned Combinatorial Libraries
Luke H. Bradley, Peter P. Thumfort, and Michael H. Hecht
Non-Protein Amino Acids in the Design of Secondary Structure Scaffolds
Radhakrishnan Mahalakshmi and Padmanabhan Balaram
Design and Synthesis of b-Peptides With Biological Activity
Marc J. Koyack and Richard P. Cheng
II. Design of Entire Proteins and Protein Complexes
Design of Miniproteins by the Transfer of Active Sites Onto Small-Size Scaffolds
François Stricher, Loïc Martin, and Claudio Vita
Consensus Design as a Tool for Engineering Repeat Proteins
Tommi Kajander, Aitziber L. Cortajarena, and Lynne Regan
Multiple Sequence Alignment as a Guideline for Protein Engineering Strategies
Alan R. Davidson
Sequence Search Methods and Scoring Functions, for the Design of Protein Structures
Hocine Madaoui, Emmanuelle Becker, and Raphael Guerois
Prediction of Protein-Protein Interaction Based on Structure
Gregorio Fernandez-Ballester and Luis Serrano
Electrostatic Design of Protein-Protein Association Rates
Gideon Schreiber, Yossi Shaul, and Kay E. Gottschalk
III. Design of Amyloidogenic Polypeptides and Amyloid Inhibitors
Peptide Model Systems for Amyloid Fiber Formation: Design Strategies and Validation Methods
Alexandra Esteras-Chopo, MarÃa Teresa Pastor, and Manuela López de la Paz
Protein Misfolding Disorders and Rational Design of Antimisfolding Agents
Lisbell D. Estrada, June Yowtak, and Claudio Soto
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: June, 2006
Pages: 300
Weight: 630g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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CUSTOMER REVIEWS
"...provides a comprehensive guide to the methods used at every stage of the protein-design process..." - ChemBioChem
"...combines a thorough theoretical foundation with detailed protocols and will be invaluable to all researchers in the area, from graduate students to senior investigators." -ChemBioChem