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Main description:
In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.
Feature:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
In Situ Hybridization Protocols, Fourth Edition, contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.
Contents:
1. Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae
Ricardo Fuentes and Juan Fernández
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2. MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos
Karen Goossens, Luc Peelman, and Ann Van Soom
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3. Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies
Eleni Dakou, Nele Vanbekbergen, Sara Corradi, Caroline R. Kemp, Erik Willems, and Luc Leyns
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4. Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian
Agnieszka Rybak-Wolf and Jordi Solana
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5. In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae
Bernard Thisse and Christine Thisse
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6. LNA-based in Situ Hybridization Detection of mRNAs in Embryos
Diana K. Darnell and Parker B. Antin
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7. Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNAâ„¢ Probe Technology
Boye Schnack Nielsen, Trine Møller, and Kim Holmstrøm
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8. MicroRNA In Situ Hybridisation in Tissue Microarrays
Julia J. Turnock-Jones and John P.C. Le Quesne
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9. Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections
Asli Silahtaroglu
 10. Sensitive and Specific in situ Hybridisation for Early Drug DiscoveryPernille A. Usher, Elisabeth D. Galsgaard, Kimberly Kruse, Jishu Wang, Berit O. Krogh, Jette Mandelbaum, and Kasper Almholt
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11. Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections
Electra Stylianopoulou, George Skavdis and Maria Grigoriou
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12. Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope
Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma
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13. Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens
Lorenzo F. Sempere
14. Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues
Neil Renwick, Pavol Cekan, Claudia Bognanni, and Thomas Tuschl
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15. Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris® RNA Fluorescence in situ Hybridization in Adherent Cells
Sally R. Coassin, Arturo V. Orjalo, Jr., Sheila J. Semaan, and Hans E. Johansson
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16. Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope
Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma
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17. Identification of Low Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia
Sergei Zolotukhin
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18. In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Kathleen T. Montone
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19. In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based Tissue Extraction from Thin Slide-mounted Sections for DNA Methylation Analysis
Lawrence S. Own and Paresh D. Patel
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20. miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH
Meiye Wu, Matthew E. Piccini, and Anup K. Singh
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21. PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples
Mustafa Fazli, Thomas Bjarnsholt, Niels Høiby, Michael Givskov, and Tim Tolker-Nielsen
PRODUCT DETAILS
Publisher: Springer (Springer New York)
Publication date: September, 2014
Pages: 230
Weight: 771g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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