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Legionella
Methods and Protocols
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Main description:

This Methods in Molecular Biology (TM) series book presents methods specifically adapted and developed for the study of distinct features of L. pneumophila. Includes materials lists, reproducible protocols, and notes on troubleshooting and pitfalls.


Contents:

Part 1. Identification and Typing of Legionella in the Environment and During Disease

1. Identification of Legionella in the Environment

Natalia A. Kozak, Claressa E. Lucas, and Jonas M. Winchell

2. Identification of Legionella in Clinical Samples

Sophe Jarraud, Ghislaine Descours, Christophe Ginevra, Gerard Lina, and Jerome Etienne

3. Sequence-Based Identification of Legionella

Rodney M. Ratcliff

4. Investigation of Outbreaks - Epidemiology

Carol Joseph

5. Outbreak Investigations and Identification of Legionella in Contaminated Water

Susanne Lee and John Lee

6. Typing Methods for Legionella

Christian Luck, Norman K. Fry, Jurgen H. Helbig, Sophie Jarraud, and Timothy G. Harrison

Part 2. Physiology and genetics of Legionella

7. Culture, Media and Handling of Legionella

Christa H. Chatfield and Nicholas P. Cianciotto

8. The Intracellular Metabolism of Legionella by Isotopologue Profiling

Klaus Heuner and Wolfgang Eisenreich

9. Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction

Carmen Buchrieser and Xavier Charpentier

10. Constructing Unmarked Gene Deletions in Legionella pneumophila

Andrew Bryan, Zachary D. Abbot, and Michele Swanson

11. Natural Biofilm Formation with Legionella pneumophila

Emilie Portier and Yann Hechard

12. Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium

Sophie Pecastaings and Christine Roques

13. Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila

Jens Jager and Michael Steinert

Part 3. Cellular microbiology of Legionella

14. Analysis of Legionella Infection by Flow Cytometry

Andre Tiaden, Aline Kessler, and Hubert Hilbi

15. Analysis of Legionella Infection Using RNAi in Drosophila Cells

Dennise A. De Jesus, Tamara J. O'Connor, and Ralph R. Isberg

16. Immunofluorescence Imaging of Legionella Infection In vitro

Stefan S. Weber and Annette Oxenius

17. Electron Microscopy of Legionella and Legionella-Infected Cells

Gary Faulkner and Raphael Garduno

18. Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages

Christine Hoffmann, Ivo Finsel, and Hubert Hilbi

19. Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System

Wenhan Zhu and Zhao-Qing Luo

20. Subcellular Localization of Legionella Dot/Icm Effectors

Adam J. Vogrin, Aurelie Mousnier, Gad Frankel, and Elizabeth L. Hartland

Part 4. Biochemical assays to study Legionella effectors and enzymes

21. Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase

Hiroki Nagai and Tomoko Kubori

22. Legionella Phospholipases Implicated in Infection - Determination of Enzymatic Activities

Kathleen Seipel and Antje Flieger

23. Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids

Stephen Weber, Stephanie Dolinsky, and Hubert Hilbi

24. Characterization of Legionella lipopolysaccharide

Christian Luck and Jurgen Helbig

Part 5. Immunity and host response against Legionella

25. The Tetrahymena and Acanthamoeba Model Systems

Sharon G. Berk and Rafael A. Garduno

26. Dictyostelium Host Response to Legionella infection: Strategies and Assays

Salvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger

27. The Caenorhabditis elegans Model of Legionella Infection

Ann Karen C. Brassinga and Costi D. Sifri

28. Infection of Nonphagocytic Host Cells by Legionella

Shandra R. Day, Costi D. Sifri, and Paul S. Hoffman

29. Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages

Christopher L. Case and Craig R. Roy

30. The Mouse as a Model for Pulmonary Legionella Infection

Juliana I. Hori and Dario Zamboni

31. Assessment of Legionella-Specific Immunity in Mice

Stefan Weber, Nicole Joller, and Annette Oxenius

32. The Guinea Pig Model of Legionnaires' Disease

Paul H. Edelstein

33. Human Susceptibility to Legionnaires Disease

William R. Berrington and Thomas R. Hawn

Part 6. Genomics, transcriptomics and proteomics of Legionella

34. cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila

Tobias Sahr and Carmen Buchrieser

35. Methods to Study Legionella Transcriptome in vitro and in vivo

Sebastian Faucher and Howard Shuman

36. Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction

Tobias Sahr and Carmen Buchrieser

37. Identification of Legionella Effectors Using Bioinformatic Approaches

Gil Segal

Part 5. Immunity and host response against Legionella

25. The Tetrahymena and Acanthamoeba Model Systems

Sharon G. Berk and Rafael A. Garduno

26. Dictyostelium Host Response to Legionella infection: Strategies and Assays

Salvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger

27. The Caenorhabditis elegans Model of Legionella Infection

Ann Karen C. Brassinga and Costi D. Sifri

28. Infection of Nonphagocytic Host Cells by Legionella

Shandra R. Day, Costi D. Sifri, and Paul S. Hoffman

29. Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages

Christopher L. Case and Craig R. Roy

30. The Mouse as a Model for Pulmonary Legionella Infection

Juliana I. Hori and Dario Zamboni

31. Assessment of Legionella-Specific Immunity in Mice

Stefan Weber, Nicole Joller, and Annette Oxenius

32. The Guinea Pig Model of Legionnaires' Disease

Paul H. Edelstein

33. Human Susceptibility to Legionnaires Disease

William R. Berrington and Thomas R. Hawn

Part 6. Genomics, transcriptomics and proteomics of Legionella

34. cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila

Tobias Sahr and Carmen Buchrieser

35. Methods to Study Legionella Transcriptome in vitro and in vivo

Sebastian Faucher and Howard Shuman

36. Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction

Tobias Sahr and Carmen Buchrieser

37. Identification of Legionella Effectors Using Bioinformatic Approaches

Gil Segal


PRODUCT DETAILS

ISBN-13: 9781627031608
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2012
Pages: 1017
Weight: 652g
Availability: Available
Subcategories: Infectious Diseases, Public Health
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