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MORE ABOUT THIS BOOK
Main description:
In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.
Contents:
1 Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation
Carl Fortin and Tamas Fuloep
2 Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing
Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa and Rafael Solana
3 Flow Cytometric Identification of Fibrocytes in the Human Circulation
Xinyuan Hu, Erin M. DeBiasi, and Erica L. Herzog
4 Experimental Approaches to Tissue Injury and Repair in Advanced Age
Aleah L. Brubaker, Stewart R. Carter, and Elizabeth J. Kovacs
5 Multicolor Digital Flow Cytometry Used in Human Translational Immunology
Samit Joshi, Subhasis Mohanty, Albert C. Shaw
6 Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates
Christine Meyer, Kristen Haberthur, Mark Asquith and Ilhem Messaoudi
7 Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry
Michael D. Leipold, Evan W. Newall, and Holden T. Maecker
8 Imaging Immunosenescence
Feng Qian and Ruth R. Montgomery
9 Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine
Daniela Frasca, Alain Diaz, and Bonnie B. Blomberg
10 Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow Cytometry
Min Sun Shin and Insoo Kang
11 Cell-Mediated Immune Response to Influenza
Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses
Janet C. McElhaney and Beth Gentleman
12 Assays for Monitoring Macroautophagy Activity in T Cells
Yair Botbol and Fernando Macian
13 Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological Tissues
Ashish R. Chauduri, Rochelle Wei, Arunabh Bhattacharya, and Ryan Hamilton
14 Monitoring the DNA Damage Response at Dysfunctional Telomeres
Rekha Rai and Sandy Chang
15 Single-Cell Analysis of T Cell Receptor ab Repertoire
Pradyot Dash, George C. Wang, and Paul G. Thomas
16 Assessment of B Cell Repertoire in Humans
Yu-Chang Wu, David Kipling, and Deborah Dunn-Walters
17 Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA Sequencing
Chen Wang, Yi Liu, Krishna M. Roskin, Katherine J.L. Jackson, Scott C. Boyd
18 Discovery of Novel MicroRNAs in Aging C. Elegans
Alexandre de Lencastre and Frank Slack
19 Analysis of DNA Methylation by Pyrosequencing
Colin Delaney, Sanjay K. Garg, and Raymond Yung
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2015
Pages: 245
Weight: 7528g
Availability: Available
Subcategories: Immunology
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