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Main description:
RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology (TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.
Contents:
1. SuperSAGE: Powerful Serial Analysis of Gene Expression
Hideo Matsumura, Naoya Urasaki, Kentaro Yoshida, Detlev H. Kruger, Gunter Kahl, and Ryohei Terauchi
2. Northern Blot Analysis for Expression Profiling of mRNAs and Small RNAs
Ankur R. Bhardwaj, Ritu Pandey, Manu Agarwal, and Surekha Katiyar-Agarwal
3. Construction of RNA-Seq Libraries from Large and Microscopic Tissues for the Illumina Sequencing Platform
Hagop S. Atamian and Isgouhi Kaloshian
4. Strand-Specific RNA-Seq Applied to Malaria Samples
Nadia Ponts, Duk-Won D. Chung, and Karine G. Le Roch
5. RNA In Situ Hybridization in Arabidopsis
Miin-Feng Wu and Doris Wagner
6. Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning
Marta Barcala, Carmen Fenoll, and Carolina Escobar
7. Detection and Quantification of Alternative Splicing Variants Using RNA-Seq
Douglas W. Bryant, Jr., Henry D. Priest, and Todd C. Mockler
8. Separating and Analyzing Sulfur-Containing RNAs with Organomercury Gels
Elisa Biondi and Donald H. Burke
9. RNAse Mapping and Quantitation of RNA Isoforms
Lakshminarayan K. Venkatesh, Olufemi Fasina, and David J. Pintel
10. Detection and Quantification of Viral and Satellite RNAs in Plant Hosts
Sun-Jung Kwon, Jang-Kyun Seo, and A.L.N. Rao
11. In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes
Xiaozhen Yao, Hai Huang, and Lin Xu
12. Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora and Fusarium Using Illumina High Throughput Deep Sequencing
Gyungsoon Park and Katherine A. Borkovich
13. Isolation and Profiling of Protein-Associated Small RNAs
Hongwei Zhao, Yifan Lii, Pei Zhu, and Hailing Jin
14. New Virus Discovery by Deep Sequencing of Small RNAs
Kashmir Singh, Ravneet Kaur, and Wenping Qiu
15. Global Assembly of Expressed Sequence Tags (ESTs)
Foo Cheung
16. Computational Analysis of RNA-Seq
Scott A. Givan, Christopher A. Bottoms, and William G. Spollen
17. Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data
Weixiong Zhang, Xuefeng Zhou, Xiang Zhou, and Jing Xia
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: May, 2012
Pages: 248
Weight: 702g
Availability: Available
Subcategories: Genetics
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