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MORE ABOUT THIS BOOK
Main description:
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Feature:
Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques
Includes detailed, step by step guidelines for the analysis of chromatin structure and its role in transcription, replication and other biological processes
Details elaborate protocols for the detection and characterization of histone modifications including acetylation, methylation and ubiquitination
Describes protocols for techniques ranging from immunohistochemical detection of chromatin modifications to genome-wide analysis of chromatin associated proteins
Acts as a comprehensive guide to the most up to date techniques dealing with all aspects of chromatin biology
Presents experimental details in an easy to follow modular format typical of the MiMB series
Back cover:
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Contents:
1. Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos
Edgar Bonte and Peter B. Becker
2. Analysis of Reconstituted Chromatin Using a Solid-Phase Approach
Raphael Sandaltzopoulos and Peter B. Becker
3. In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins
Sherry L. Winter, Philip Wong, and Mark G. Alexandrow
4. DNA Methyltransferase Probing of Chromatin Structure within Populations and on Single Molecules
Carolina Pardo, Scott A. Hoose, Santhi Pondugula, and Michael P. Kladde
5. Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues
Takashi Furusawa and Michael Bustin
6. Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin
Eisuke Gotoh
7. Analysis of DNA Topology in Yeast Chromatin
Randall H. Morse
8. Preparation and Analysis of Uniquely Positioned Mononucleosomes
Daria A. Gaykalova, Olga I. Kulaeva, Vladimir A. Bondarenko, and Vasily M. Studitsky
9. Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy
Michael J. Kruhlak, Arkady Celeste, and André Nussenzweig
10. Methods to Study Transcription-Coupled Repair in Chromatin
Hélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera
11. Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs)
Toshiki Tanaka, Dorota Halicka, Frank Traganos, and Zbigniew Darzynkiewicz
12. Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay
Lori A.Hazlehurst
13. Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes
Melanie Carless
14. In Vitro Replication Assay with Mammalian Cell Extracts
Wasia Rizwani and Srikumar P. Chellappan
15. Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain Reaction
Melanie Carless
16. Biochemical Analysis of Arginine Methylation in Transcription
Marc Tini, Hina Naeem, and Joseph Torchia
17. In Vitro Histone Demethylase Assays
Kenji Kokura and Jia Fang
18. Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation
Shrikanth S. Gadad, Jayasha Shandilya, Venkatesh Swaminathan, and Tapas K. Kundu
19. Histone Deacetylase Activity Assay
Zhigang Yuan, Natalie Rezai-Zadeh, Xiaohong Zhang, and Edward Seto
20. In vitro and in vivo Assays for Studying Histone Ubiquitination and Deubiquitination
Ling Zhai, Heui-Yun Joo, and Hengbin Wang
21. Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Jaya Padmanabhan
22. Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications in vivo
Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan
23. ChIP on Chip Assays: Genomewide Analysis of Transcription Factor Binding and Histone Modifications
Smitha Pillai and Srikumar P. Chellappan
24. ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays
Sean J. Yoder and Steven A. Enkemann
25. Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
Partha P. Banerjee and Shankar Jagadeesh
26. Detecting ATM Dependent Chromatin Modification in DNA Damage a
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: March, 2009
Pages: 416
Weight: 1106g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry, Genetics
From the same series
CUSTOMER REVIEWS
From the reviews of the second edition: “This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of nucleosomes, the analysis of basic chromatin structure, the covalent modification of histones and the analysis of chromatin function. … In summary, this is a very useful text, with lots of strengths … .” (Ray Waters, Human Genetics, Vol. 126, 2009)