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MORE ABOUT THIS BOOK
Main description:
The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, C. elegans: Methods and Applications, Second Edition aims to ensure successful results in the further study of this dynamic field.
Feature:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, C. elegans: Methods and Applications, Second Edition aims to ensure successful results in the further study of this dynamic field.
Contents:
1. Library Construction for Mutation Identification by Whole-Genome Sequencing
Harold E. Smith
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2. Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes
Eric S. Haag and Cristel G. Thomas
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3. Genetic Methods for Cellular Manipulations in C. elegans
Menachem Katz
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4. A Fusion PCR Method for Expressing Genetic Tools in C. elegansÂ
Yifat Eliezer and Alon Zaslaver
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5. Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI)Christian Frøkjær-Jensen
 6. Creating Genome Modifications in C. elegans using the CRISPR/Cas9 SystemJohn A. Calarco and Ari E. FriedlandÂ
7. Observing and Quantifying Fluorescent Reporters
Michael Hendricks
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8. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior
Caspar Glock, Jatin Nagpal, Alexander Gottschalk
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9. Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans
Nicholas F Trojanowski and Christopher Fang-Yen
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10. High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegansLaura Manning and Janet Richmond
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11. Electron Tomography Methods for C. elegans
David H. Hall and William J. Rice
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12. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans
Ross C. Lagoy and Dirk R. Albrecht
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13. Tracking Single C. elegans using a USB Microscope on a Motorized Stage
Eviatar I. Yemini and André E.X. Brown
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14. An Imaging System for C. elegans Behavior
Matthew A. Churgin and Christopher Fang-Yen
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15. A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae
Maria C. Ow and Sarah E. Hall
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16. Sampling and Isolation of C. elegans from the Natural Habitat
Nausicaa Poullet and Christian Braendle
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17. A Primer on Prototyping
Dylan Lynch and David Biron
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18. A Primer on Quantitative Modeling
Iulia Neagu and Erel Levine
PRODUCT DETAILS
Publisher: Springer (Springer New York)
Publication date: October, 2015
Pages: 222
Weight: 762g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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